Janelia Fluor® 552, NHS ester
Tocris Bioscience | Catalog # 8947
Key Product Details
Description
Wavelength
Product Description
Key Information: Janelia Fluor® 552, NHS ester is a yellow cell-permeable fluorogenic dye, supplied with an NHS ester reactive group for the labeling of primary amines. Also chemigenetic calcium indicator for imaging Ca2+ mediated responses. Upon self-labeling in WHaloCaMP modular biosensors, fluorescence output is modulated by photoinduced electron transfer (PET) to a protein based quencher.Application: NHS ester can be converted to relevant substrate for use in self-labeling tag systems. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Also in vivo functional imaging of Ca2+ responses (flies/mice); multiplexed functional imaging of neurons and astrocytes in zebrafish larvae; quantification of Ca2+ concentration using fluorescence lifetime imaging microscopy (FLIM).
Properties and Photophysical Data: Stable to fixation. Cell permeable. Excitation and emission maxima (λ) are 552 nm and 575 nm, respectively; quantum yield = 0.83; extinction coefficient = 129,000 M-1cm-1, KL-Z = 0.70.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Yellow |
| λabs | 552 nm |
| λem | 575 nm |
| Extinction Coefficient (ε) | 129000 M-1cm-1 |
| Quantum Yield (φ) | 0.83 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM), WHaloCaMP |
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Product Specifications for Janelia Fluor® 552, NHS ester
Molecular Weight
Formula
Storage
Purity
Chemical Name
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 5.88 | 10 |
Preparing Stock Solutions for Janelia Fluor® 552, NHS ester
The following data is based on the product molecular weight 587.54.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 17.02 mL | 85.10 mL | 170.20 mL |
| 0.5 mM | 3.40 mL | 17.02 mL | 34.04 mL |
| 1 mM | 1.70 mL | 8.51 mL | 17.02 mL |
| 5 mM | 0.34 mL | 1.70 mL | 3.40 mL |
Calculators
Background References
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Product Documents for Janelia Fluor® 552, NHS ester
Certificate of Analysis
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Product Specific Notices for Janelia Fluor® 552, NHS ester
For research use only
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