Janelia Fluor® 630b, NHS ester

Tocris Bioscience | Catalog # 8155

Blinking fluorescent dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM) including SMLM
Tocris Bioscience

Key Product Details

Description

Blinking fluorescent dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM) including SMLM

Wavelength

Excitation = 654 nm, Emission = 671 nm
View all Amine-Reactive Janelia Fluor Dyes »

Product Description

Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.

Key Information: Janelia Fluor® 630b, NHS ester is a spontaneously blinking fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for characterizing the physical properties of dense biomolecule condensates and complex macromolecular assemblies.

Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).

Properties and Photophysical Data: excitation and emission maxima (λ) are 654 nm and 671 nm, respectively.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 630b, NHS ester Dye Spectra
λabs 654 nm
λem 671 nm

Spectra Viewer

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Product Specifications for Janelia Fluor® 630b, NHS ester

Molecular Weight

735.90

Formula

C35H37N3O9S2Si

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

2,5-Dioxopyrrolidin-1-yl 5,5-dimethyl-3,7-bis(3-(methylsulfonyl)azetidin-1-yl)-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxylate

InChI Key

BTRXQVXFPGRVHI-UHFFFAOYSA-N

SMILES

C[Si]1(C2=CC(N3CC(S(C)(=O)=O)C3)=CC=C2C4(C5=CC=C(N6CC(C6)S(C)(=O)=O)C=C51)C7=C(CO4)C=CC(C(ON8C(CCC8=O)=O)=O)=C7)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 630b, NHS ester

Certificate of Analysis

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Product Specific Notices for Janelia Fluor® 630b, NHS ester

For research use only

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