Janelia Fluor® 635, NHS ester

Tocris Bioscience | Catalog # 6419

Red dye (Reactive group: NHS ester). Application: flow cytometry, confocal microscopy, super resolution microscopy. Suitable for live cell imaging
Tocris Bioscience

Key Product Details

Description

Red dye (Reactive group: NHS ester). Application: flow cytometry, confocal microscopy, super resolution microscopy. Suitable for live cell imaging

Alternative Names

JF 635,NHS

Wavelength

Excitation = 635 nm, Emission = 652 nm
View all Amine-Reactive Janelia Fluor Dyes »

Product Description

Key Information:

Janelia Fluor® 635, NHS ester is a red fluorogenic fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Cell permeable.

Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 635 nm and 652 nm, respectively; quantum yield = 0.56; extinction coefficient = 167,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); A280 correction factor = 0.0524; lactone-zwitterion equilibrium constant (KL-Z) <0.0001. Exhibits low nonspecific background staining.

Please see the protocol for further information on protein/antibody labeling and conjugation.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer custom conjugation antibody services.

HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

This product has been recently renamed. The previous name for this product was Janelia Fluor® 635, SE.

Scientific Data Images for Janelia Fluor® 635, NHS ester

Applications on Mitochondria for Janelia Fluor® 635, NHS ester

Applications on mitochondria for Janelia Fluor® 635, NHS ester.

Application of Janelia Fluor® 635, NHS ester dye on mitochondria; Left: Mitochondria were labeled with anti-TOM20 primary antibodies. The secondary antibodies were conjugated to JF 635 dye. Maximum intensity projection of confocal z-stack, recorded on an Olympus FV1200 confocal microscope. Right: Enlarged portion of left-hand image. Images kindly provided by Dr Kirstin Elgass, Monash University.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 635, NHS ester Dye Spectra
Emission Color Red
Brightness
λabs 635 nm
λem 652 nm
Extinction Coefficient (ε) 167,000 M-1cm-1
Quantum Yield (φ) 0.56
Closest Laser line 635 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.0524
Application Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry
Alternative For Alexa Fluor® 633, Atto 633, CF633

Trademarks and registered trademarks are the property of their respective owners.

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Product Specifications for Janelia Fluor® 635, NHS ester

Molecular Weight

629.69

Formula

C33H29F2N3O6Si

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-7-[1-(3-fluoroazetidinyl)]-9-silaanthracen-2(9H)-ylidene]-3-fluoroazetidinium, inner salt

PubChem ID

132470914

InChI Key

YVKNNZJUJGADIJ-UHFFFAOYSA-N

SMILES

O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)C4)C=C3[Si]5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 62.97 100

Preparing Stock Solutions for Janelia Fluor® 635, NHS ester

The following data is based on the product molecular weight 629.69.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.59 mL 7.94 mL 15.88 mL
5 mM 0.32 mL 1.59 mL 3.18 mL
10 mM 0.16 mL 0.79 mL 1.59 mL
50 mM 0.03 mL 0.16 mL 0.32 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 635, NHS ester

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 635, NHS ester

For research use only

Citations for Janelia Fluor® 635, NHS ester

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