Janelia Fluor® 635b, Maleimide

Tocris Bioscience | Catalog # 8156

Blinking fluorescent dye (Reactive group: maleimide). Application: super resolution microscopy (SRM) including SMLM
Tocris Bioscience

Key Product Details

Description

Blinking fluorescent dye (Reactive group: maleimide). Application: super resolution microscopy (SRM) including SMLM

Wavelength

Excitation = 651 nm, Emission = 667 nm
View all Spontaneously Blinking Janelia Fluor dyes »

Product Description

Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.

Key Information: Janelia Fluor® 635b, Maleimide is a spontaneously blinking fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity) for the labeling of protein. Suitable to characterize the physical properties of dense biomolecule condensates and complex macromolecular assemblies.

Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).

Properties and Photophysical Data: derived from Janelia Fluor® 635, Maleimide (Cat. No. 8027). Excitation and emission maxima (λ) are 651 nM and 667 nM, respectively.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

Scientific Data Images for Janelia Fluor® 635b, Maleimide

Application of Janelia Fluor® 635b, Maleimide.

Application of Janelia Fluor® 635b, Maleimide.

3D SMLM map of a FUS coacervate aged for 12 h containing 20 nM FUSA2C-JF635b; inset shows brightfield image; scale bar = 4 μm; n = 31,123 total localizations; colors indicate localization density within a 100 nm radius sphere: black = 1; orange 2–10; green = 11–20; cyan = 21–40; magenta = 41–100. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. bioRxiv. PMID: 38766149

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 635b, Maleimide Dye Spectra
λabs 651 nm
λem 667 nm

Spectra Viewer

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Product Specifications for Janelia Fluor® 635b, Maleimide

Molecular Weight

640.76

Formula

C35H34F2N4O4Si

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

N-(2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-3,7-bis(3-fluoroazetidin-1-yl)-5,5-dimethyl-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxamide

InChI Key

CVIGZVOMUSQNLV-UHFFFAOYSA-N

SMILES

C[Si]1(C)C2=CC(N3CC(F)C3)=CC=C2C4(OCC5=C4C=C(C(NCCN6C(C=CC6=O)=O)=O)C=C5)C7=CC=C(N8CC(F)C8)C=C71

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 635b, Maleimide

Certificate of Analysis

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Product Specific Notices for Janelia Fluor® 635b, Maleimide

For research use only

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