Janelia Fluor® 646, Haloalkane
Tocris Bioscience | Catalog # 8809
Key Product Details
Description
Wavelength
Product Description
Key Information: Janelia Fluor® 646, Haloalkane is a red cell-permeable fluorogenic fluorescent dye with a chloroalkane handle.Application: Live-cell imaging as a self-labeling tag substrate. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Stable to fixation. Cell permeable.
Properties and Photophysical Data: Janelia Fluor® 646, Haloalkane shows a high degree of fluorogenicity. Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); lactone-zwitterion equilibrium constant (KL-Z) = 0.0012. Exhibits low non-specific background staining.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Red |
| Brightness | |
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152.000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 640 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM) |
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Product Specifications for Janelia Fluor® 646, Haloalkane
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.02 | 10 |
Preparing Stock Solutions for Janelia Fluor® 646, Haloalkane
The following data is based on the product molecular weight 702.36.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 14.24 mL | 71.19 mL | 142.38 mL |
| 0.5 mM | 2.85 mL | 14.24 mL | 28.48 mL |
| 1 mM | 1.42 mL | 7.12 mL | 14.24 mL |
| 5 mM | 0.28 mL | 1.42 mL | 2.85 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Grimm A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 2017 PMID: 28869757
Product Documents for Janelia Fluor® 646, Haloalkane
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Janelia Fluor® 646, Haloalkane
For research use only
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