Janelia Fluor® 646, SE

Catalog # Availability Size / Price Qty
6148/1
Janelia Fluor® 646, SE | CAS No. 1811539-59-9 | Amine-Reactive Janelia Fluor Dyes
2 Images
Description: Red dye (Reactive group: NHS ester). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging
Alternative Names: JF 646, NHS

Chemical Name: 1-[7-(1-Azetidinyl)-10-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt

Product Details
Reviews

Biological Activity

Janelia Fluor® 646, SE is a fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Can be multiplexed for two colour imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Also suitable for flow cytometry. Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54, Max. extinction coefficient = 152,000 M-1cm-1 (measured in TFE plus 0.1% TFA); A280 correction factor is 0.19.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We recommend that stock solutions of this dye are prepared in anhydrous DMF.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Scientific Data

Application of Janelia Fluor® 646, SE in Cardiac Tissue. Super-resolution image (dSTORM) of muscle tissue from the left ventricle of an adult pig heart. JF646 (Catalog # 6148) was used to label collagen VI, which is present on the cell plasma membrane, and imaged with a 642 nm excitation laser. A clearer structure is achieved in comparison with the widefield image (top right). Scale bar 2 μm.Image kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Alex Clowsley and Anna Meletiou.

Optical Data for Janelia Fluor® 646, SE

Janelia Fluor<sup>®</sup> 646, SE Dye Spectra
Emission Color Red
Brightness
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 640 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.19
Application Confocal imaging, dSTORM, STED, Flow Cytometry
Alternative For Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data

M.Wt:
593.71
Formula:
C33H31N3O6Si
Solubility:
Soluble to 5 mM in DMSO and to 50 mM in DMF
Storage:
Store at -20°C
CAS No:
1811539-59-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Complete Your Experiment

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

Or select another batch:
Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Molarity Calculator

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

FAQs

No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for Janelia Fluor® 646, SE

There are currently no reviews for this product. Be the first to review Janelia Fluor® 646, SE and earn rewards!

Have you used Janelia Fluor® 646, SE?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.