Janelia Fluor® 646 Tre

Tocris Bioscience | Catalog # 8967

Red fluorescent trehalose probe for live mycobacteria imaging
Tocris Bioscience
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Key Product Details

Description

Red fluorescent trehalose probe for live mycobacteria imaging

Wavelength

Excitation = 646 nm, Emission = 664 nm
View all Fluorescent Probes for Imaging Bacteria »

Product Description

Key information: Janelia Fluor® 646 Tre is a red fluorescent probe for imaging Mycobacterium tuberculosis, after metabolic incorporation of the trehalose component in the lipid-rich cell envelope.

Used for: imaging Mycobacterium tuberculosis in TB drug susceptibility testing.

Application: fluorescent microscopy and flow cytometry.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Emission Color Red
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Correction Factor 280 0.19
Application Bacterial imaging

Spectra Viewer

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Product Specifications for Janelia Fluor® 646 Tre

Molecular Weight

819.94

Formula

C41H49N3O13Si

Storage

Store at -20°C

Purity

≥95%

Chemical Name

2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((((2R,3S,4S,5R,6R)-3,4,5-trihydroxy-6-(((2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)tetrahydro-2H-pyran-2-yl)methyl)carbamoyl)benzoate

InChI Key

BZQLCLILAFFJOH-KEVSEYCPSA-N

SMILES

O[C@H]1[C@@H]([C@H](O[C@@H]([C@@H]1O)O[C@@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CNC(C3=CC=C(C([O-])=O)C(C(C(C=CC(N4CCC4)=C5)=C5[Si]6(C)C)=C7C6=C/C(C=C7)=[N+]8CCC\8)=C3)=O)O)O)O)CO)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 8.2 10

Preparing Stock Solutions for Janelia Fluor® 646 Tre

The following data is based on the product molecular weight 819.94.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 12.20 mL 60.98 mL 121.96 mL
0.5 mM 2.44 mL 12.20 mL 24.39 mL
1 mM 1.22 mL 6.10 mL 12.20 mL
5 mM 0.24 mL 1.22 mL 2.44 mL

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Background References

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There are currently no references for this product.

Product Documents for Janelia Fluor® 646 Tre

Certificate of Analysis

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Product Specific Notices for Janelia Fluor® 646 Tre

For research use only

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