Janelia Fluor® 669, SE
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Chemical Name: 1-[7-(1-Azetidinyl)-10-[2-Carboxy-5-[(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethyl]thio-3,4,6-trifluorophenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt
Purity: ≥90%
Biological Activity
Janelia Fluor® 669, SE is a red fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 669 nm; emission maximum = 682 nm; Quantum yield = 0.37; Extinction coefficient = 116,000 M-1cm-1; Correction factor = 0.0430. Retains 97% fluorescence after 30 bleaching cycles.Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Scientific Data
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Applications on Tubulin for Janelia Fluor® 669, SE. Application of Janelia Fluor® 669, SE dye on Tubulin; Left: Microtubules were labeled with anti-alpha -tubulin primary antibodies. The secondary antibodies were conjugated to Janelia Fluor® 669, SE. Maximum intensity projection of confocal z-stack, recorded with an Olympus FV1200 confocal microscope, using 638 nm laser line for JF 669. Right: Enlarged portion of left-hand image. Images kindly provided by Dr Kirstin Elgass, Monash University.
Optical Data for Janelia Fluor® 669, SE
| Emission Color | Far Red |
|---|---|
| Brightness | |
| λabs | 669 nm |
| λem | 682 nm |
| Extinction Coefficient (ε) | 116,000 M-1cm-1 |
| Quantum Yield (φ) | 0.37 |
| Closest Laser line | 640 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.043 |
| Application | Confocal fluorescent imaging, SRM |
| Alternative For | Alexa Fluor® 660, Atto 655, Atto 665, CF660R |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
Background References
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A general method to fine-tune fluorophores for live-cell and in vivo imaging.
Grimm et al.
Nat.Methods, 2017;14:987 -
General synthetic method for Si-Fluoresceins and Si-Rhodamines.
Grimm et al.
ACS Cent.Sci., 2017;3:975 -
A general method to optimize and functionalize red-shifted rhodamine dyes.
Grimm et al.
Nat.Methods, 2020;17:815
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