Janelia Fluor® 669, SE

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Janelia Fluor® 669, SE | CAS No. 2127150-20-1 | Amine-Reactive Janelia Fluor Dyes
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Description: Far red dye (Reactive group: NHS ester). Applications: super resolution microscopy e.g. dSTORM & STED, confocal and live cell imaging
Alternative Names: JF 669, NHS

Chemical Name: 1-[7-(1-Azetidinyl)-10-[2-Carboxy-5-[(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethyl]thio-3,4,6-trifluorophenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt

Purity: ≥90%

Product Details

Biological Activity

Janelia Fluor® 669, SE is a red fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 669 nm; emission maximum = 682 nm; Quantum yield = 0.37; Extinction coefficient = 116,000 M-1cm-1; Correction factor = 0.0430. Retains 97% fluorescence after 30 bleaching cycles.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Scientific Data

Confocal Microscopy Applications on Tubulin for Janelia Fluor® 669, SE View Larger

Applications on Tubulin for Janelia Fluor® 669, SE. Application of Janelia Fluor® 669, SE dye on Tubulin; Left: Microtubules were labeled with anti-alpha -tubulin primary antibodies. The secondary antibodies were conjugated to Janelia Fluor® 669, SE. Maximum intensity projection of confocal z-stack, recorded with an Olympus FV1200 confocal microscope, using 638 nm laser line for JF 669. Right: Enlarged portion of left-hand image. Images kindly provided by Dr Kirstin Elgass, Monash University.

Optical Data for Janelia Fluor® 669, SE

Janelia Fluor<sup>®</sup> 669, SE Dye Spectra
Emission Color Far Red
λabs 669 nm
λem 682 nm
Extinction Coefficient (ε) 116,000 M-1cm-1
Quantum Yield (φ) 0.37
Closest Laser line 640 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.043
Application Confocal fluorescent imaging, SRM
Alternative For Alexa Fluor® 660, Atto 655, Atto 665, CF660R

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

Soluble to 20 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

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