Jess Automated Western Blot System

Meet Jess, your protein analysis problem solver. Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. Just load your samples and reagents into the microplate, and Jess does the rest. She separates your protein by size and precisely manages antibody additions, incubations, washes and even the detection steps. Come back to fully analyzed and quantitated results in just 3 hours. With Jess’s chemiluminescent detection you’ll get picogram-level sensitivity, letting you maximize the data you get from your sample. Go further with multiplexing—her fluorescent detection gets all the information you need in one shot. Best of all, you get reliable and reproducible results with target normalization to the amount of protein loaded.

How does Simple Western work?

Features

Fluorescence Detection

Why bother stripping and reprobing? Maximize your time and sample, and get the information you need in one shot, by multiplexing.

Jess’s fluorescent detection capabilities enable two-color protein detection for multiplexing. Detection of Stat3 (green) and phospho-Stat3 (red) in K562 cell lysate. Lane 1: ladder; Lane 2: phospho-Stat3; Lane 3: Stat 3; Lane 4: phospho-Stat3/Stat3.

Chemiluminescence Detection

Working with low abundance targets or precious samples? Chemiluminescent detection gives you picogram-level sensitivity, letting you maximize the data you get from your sample.

Explore chemiluminescent Westerns with lane view, or dive deeper with quantitative electropherograms.

Protein Normalization

Jess gives you an easy way to see if your samples contain a consistent protein load—just load her in-capillary protein normalization reagent into her assay plate and she’ll take care of the rest. Her proprietary fluorescent protein normalization reagent measures proteins immobilized in the same capillary as your immunoassay. Best of all, Jess’s multiple detection capabilities enable two-color protein detection for multiplexing and chemiluminescent detection, on top of protein normalization.

With protein normalization, you can take your protein load comparison and transform your data to effectively normalize your samples, increasing your confidence in your data interpretation.

Blot Imaging

Still doing traditional Westerns? Snap! Get the picture with Jess’s blot imaging system.

1. Image traditional chemiluminescent blot membranes 2. Chemiluminescent Western blots imaged on Jess