Simple Western Models
Evolve Your Protein Analysis With Simple Western Technology
Which Simple Western System Is Right for You?
| System | Jess | Abby | Leo |
| Flexible, Multiplexing | Budget-Friendly | High throughput, Quantitative | |
| Separation Mode | Size | Size | Size |
| Max capillaries per run | 25 | 25 | 100 |
| Run time* | <3 hours RePlex: 5 hours | <3 hours RePlex: 5 hours | 3 hours RePlex: 5 hours |
| Minimal sample volume requirements | 3 µl/well | 3 µl/well | 3 µl/well |
| Detection Modes | Chemiluminescence and Fluorescence (NIR & IR) | Chemiluminescence | Chemiluminescence and Fluorescence (NIR & IR) |
| RePlex | Yes | Yes | Yes |
| Dimensions (H x W x D) | 0.33 m x 0.36 m x 0.52 m (1.07' x 1.15' x 1.71') | 0.36 m x 0.36 m x 0.57 m (1.08' x 1.08' x 1.71') | 0.75 m x 0.70 m x 0.73 m (2.5’ x 2.3’ x 2.4’) |
| Weight | 23 kg (50 lbs) | 21 kg (46 lbs) | 136 kg (300 lbs) |
| Part Number | 004-650 | 004-680 | 004-550 |
NanoPro 1000
| System | NanoPro 1000 |
| Separation Mode | Charge |
| Max capillaries per run | 96 |
| Max run time | 11 hours |
| Sample volume required | 5–12 µL/well |
| Chemiluminescence | Yes |
| Fluorescence | No |
| RePlex | No |
| Sample cooling | 3 ºC |
| Dimensions (H x W x D) | 94 x 94 x 61 cm |
| Weight | 82 kg (180 lb) |
| Part number | 004-109 |
Jess & Abby | Leo | NanoPro 1000 | |||||
| Mode | Chemi | Fluor | Total Protein | Chemi | Fluor | Total Protein | Charge |
| Sample Required | 0.3-1.2 µg | 2-4 µg | 0.3-1.2 µg | 0.3-1.2 µg | 2-4 µg | 0.3-1.2 µg | 0.3-1.2 µg |
| Size Range | Molecular weight (MW) ladders range from 2–440 kDa | Molecular weight (MW) ladders range from 2–440 kDa | Widest gradient ranges from pl 3 to pl 10 | ||||
| Sizing CV | <10% | <10% | <10% | ||||
| Intra-assay peak area CV | <15% | <15%1 | <20% | ||||
| Inter-assay peak area CV | <20%3 | <20% | -- | <15% | <15%2 | -- | <20% |
| Intra-assay quantitation CV | <20%4 | <20% | -- | <10%4 | <20% | -- | <20% |
| Inter-run quantitation CV | -- | -- | -- | <10%4 | -- | -- | -- |
| Inter-instrument quantitation CV | -- | -- | -- | <10%4 | -- | -- | -- |
| Resolution (± percent difference in MW) | ± 10% for MW >20KDa ± 15-20% for MW <20KDa | ± 10% for MW >20KDa ± 15-20% for MW <20KDa | ± 1 pl units | ||||
| Dynamic Range | 3-4 logs | 3-4 logs | 2-3 logs | 3-4 logs | 3-4 logs | 2-3 logs | 3 logs |
| Sensitivity | Low pg | High pg | ng | Low pg | High pg | ng | Low pg |
+Sample Required is 3 μL/well for Leo, Jess, and Abby systems, and 12 μL/well for NanoPro 1000 system.
1-Based on model assay system with signal to noise ratio > 250, n= 96, Leo uses cartridge correction.
2-Based on model assay system, signal to noise ratio >250, n=3, Leo uses cartridge correction.
3-Inter-assay CV is with system control
4-%CV of calculated concentration generated using a standard curve for all samples above LLOQ. Leo uses cartridge correction.
Trade up your Peggy Sue or Sally Sue System for the new Leo system and maximize throughput and process more samples in a day.
The Peggy Sue and Sally Sue Systems are discontinued as of Dec 31st, 2024 with a last time purchase date of June 30th, 2025. ProteinSimple will continue service and support of these systems through Dec 31st, 2029. If you are interested in acquiring Simple Western systems after this date, you will be able to purchase our Jess, Abby and NanoPro 1000 systems as well as our newest system, Leo. Our new Leo System enables fast, simple and sensitive protein quantification and detection, processing up to 100 capillaries per run in as little as 3 hours.
The Wes System has been discontinued as of July 30th, 2021. ProteinSimple will continue service and support of the Wes platform through July 30th, 2026. Upgrade to Leo, Jess, or Abby to have all the functionality of Wes with additional features like RePlex.