JFX™ 554, Maleimide

Tocris Bioscience | Catalog # 8160

Deuterated Janelia Fluor® dye (Reactive group: Maleimide). Application: confocal microscopy, super-resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Tocris Bioscience

Key Product Details

Description

Deuterated Janelia Fluor® dye (Reactive group: Maleimide). Application: confocal microscopy, super-resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging

Wavelength

Excitation = 554 nm, Emission = 576 nm
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Product Description

Key Information:

JFX™ 554, Maleimide is a yellow fluorescent deuterated Janelia Fluor® dye; supplied with a Maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.

Application: Suitable for confocal microscopy, super-resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: JFX™ 554, Maleimide shows enhanced brightness, photostability and chromostability. Maleimide can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 554 nm and 576 nm, respectively; quantum yield = 0.8; extinction coefficient = 104,600 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 4.97.

This product is provided in a DMSO solution at a concentration of 1 mM.

Please see the product protocol for further information and a guide to protein/antibody labeling.

We also offer custom conjugation antibody services.

HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

JFX™ is a trademark owned by the Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

JFX™ 554, Maleimide Dye Spectra
λabs 554 nm
λem 576 nm
Extinction Coefficient (ε) 104,000 M-1cm-1
Quantum Yield (φ) 0.8
Reactive Group Maleimide

Spectra Viewer

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Product Specifications for JFX™ 554, Maleimide

Molecular Weight

734.78

Formula

C35H16D16N4O6.CF3CO2H

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

4-((2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)-2-(3-(pyrrolidin-1-ium-1-ylidene-d8)-6-(pyrrolidin-1-yl-d8)-3H-xanthen-9-yl)benzoate trifluoroacetate

InChI Key

ODPVJSHHEVQADP-CXZJOTLHSA-N

SMILES

[2H]C(C([2H])([2H])C([2H])([2H])C/1([2H])[2H])([2H])[N+]1=C2C=CC3=C(C4=CC(C(NCCN5C(C=CC5=O)=O)=O)=CC=C4C([O-])=O)C6=CC=C(N7C([2H])([2H])C([2H])([2H])C([2H])([2H])C7([2H])[2H])C=C6OC3=C/2.FC(F)(C(O)=O)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solubility Soluble in DMSO (Supplied pre-dissolved in DMSO, 1 mM)

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Background References

References are publications that support the biological activity of the product.

Product Documents for JFX™ 554, Maleimide

Certificate of Analysis

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Product Specific Notices for JFX™ 554, Maleimide

For research use only

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