JFX™ 650, NHS ester

Tocris Bioscience | Catalog # 8163

Deuterated Janelia Fluor® dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Tocris Bioscience

Key Product Details

Description

Deuterated Janelia Fluor® dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging

Wavelength

Excitation = 650 nm, Emission = 667 nm
View all Amine-Reactive Janelia Fluor Dyes »

Product Description

Key Information:

JFX™ 650, NHS ester is a red fluorescent deuterated Janelia Fluor® dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.

Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: JFX™ 650, NHS ester shows enhanced brightness, photostability and chromostability. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 650 nm and 667 nm, respectively; quantum yield = 0.53; extinction coefficient = 17,600 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.015.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We also offer custom conjugation antibody services.

HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

JFX™ is a trademark owned by the Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

JFX™ 650, NHS ester Dye Spectra
λabs 650 nm
λem 667 nm
Extinction Coefficient (ε) 17,600 M-1cm-1
Quantum Yield (φ) 0.53
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Application Confocal Microscopy, dSTORM, STED, Flow Cytometry

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Product Specifications for JFX™ 650, NHS ester

Molecular Weight

637.86

Formula

C35H19D16N3O6Si

Storage

Store at -20°C

Purity

≥85% (HPLC)

Chemical Name

2-(5,5-Dimethyl-3-(pyrrolidin-1-ium-1-ylidene-d8)-7-(pyrrolidin-1-yl-d8)-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate

CAS Number

2396479-84-6

PubChem ID

153290020

InChI Key

OJCIIHUZGDFMHK-UDNRTFQASA-N

SMILES

[2H]C(C([2H])([2H])C([2H])([2H])C/1([2H])[2H])([2H])[N+]1=C2C=CC3=C(C4=CC(C(ON5C(CCC5=O)=O)=O)=CC=C4C([O-])=O)C6=CC=C(N7C([2H])([2H])C([2H])([2H])C([2H])([2H])C7([2H])[2H])C=C6[Si](C)(C)C3=C/2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for JFX™ 650, NHS ester

Certificate of Analysis

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Product Specific Notices for JFX™ 650, NHS ester

For research use only

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