JH 295

Key Product Details

Product Description

JH 295 is an irreversible Nek2 inhibitor (IC₅₀=770 nM). Inhibits Nek2 via alkylation of residue Cys22. Displays 5-fold selectivity for Nek2 over Cdk1. Exhibits no significant effect on Aurora B or Plk1 kinases, bipolar spindle assembly or spindle assembly checkpoint. Cell permeable.

Product Specifications for JH 295

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for JH 295

The following data is based on the product molecular weight 320.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.12 mL	15.61 mL	31.22 mL
5 mM	0.62 mL	3.12 mL	6.24 mL
10 mM	0.31 mL	1.56 mL	3.12 mL
50 mM	0.06 mL	0.31 mL	0.62 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 4 publications citing the usage of this product.

• Henise Irreversible Nek2 kinase inhibitors with cellular activity. J.Med.Chem. 2011 PMID: 21627121

Product Documents for JH 295