JQ1-FITC

Tocris Bioscience | Catalog # 7722

Fluorescent BET bromodomain probe; suitable for use in TR-FRET
Tocris Bioscience

Key Product Details

Description

Fluorescent BET bromodomain probe; suitable for use in TR-FRET

Wavelength

Excitation = 495 nm, Emission = 525 nm
View all Fluorescent Probes »

Product Description

JQ1-FITC is a fluorescent BET bromodomain probe. It binds with high affinity to BRD4(BD1) and BRD4(BD2) (KD,app values are 6.5 nM and 5.8 nM, respectively, using GST-tagged BRD4 proteins measured by CoraFluor™ TR-FRET assay; 58.7 nM for BRD4(BD1) in a fluorescence anisotropy assay). Excitation and emission maxima (λ) are 495 nm and 525 nm.

Optical Data

JQ1-FITC Dye Spectra
Emission Color Green
λabs 495 nm
λem 525 nm
Extinction Coefficient (ε) 7500 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Application TR-FRET, Fluorescence polarization assays
Alternative For FITC

Spectra Viewer

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Product Specifications for JQ1-FITC

Molecular Weight

1106.58

Formula

C50H50ClN7O9S2.CF3CO2H

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

(S)-5-(3-(1-(4-(4-Chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)thioureido)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid trifluoroacetate

InChI Key

QRVVCZSOSIMDNN-LMDYQTDMSA-N

SMILES

O=C(NCCCOCCOCCOCCCNC(NC1=CC=C(C(C(O)=O)=C1)C2=C(C(OC3=C2C=CC(O)=C3)=C4)C=CC4=O)=S)C[C@@H]5N=C(C6=CC=C(Cl)C=C6)C7=C(SC(C)=C7C)N8C5=NN=C8C.OC(C(F)(F)F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.07 10

Preparing Stock Solutions for JQ1-FITC

The following data is based on the product molecular weight 1106.58.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 9.04 mL 45.18 mL 90.37 mL
0.5 mM 1.81 mL 9.04 mL 18.07 mL
1 mM 0.90 mL 4.52 mL 9.04 mL
5 mM 0.18 mL 0.90 mL 1.81 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for JQ1-FITC

Certificate of Analysis

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Product Specific Notices for JQ1-FITC

For research use only

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