JW 67

Tocris Bioscience | Catalog # 4651

Wnt pathway inhibitor; induces degradation of active β-catenin
Tocris Bioscience
Discontinued Product
4651 has been discontinued. View all Other Wnt Signaling Inhibitors products.

Key Product Details

Description

Wnt pathway inhibitor; induces degradation of active β-catenin
View all Other Wnt Signaling Inhibitors »

Product Description

JW 67 is an inhibitor of canonical Wnt pathway signaling (IC50 = 1.17 μM); targets the β-catenin destruction complex (GSK-3β/AXIN/APC) to induce β-catenin degradation. Selective for the canonical Wnt pathway over the Sonic hedgehog (Shh) and NF-κB pathways. Blocks G1/S cell cycle progression in colorectal cancer (CRC) cell lines (GI50 = 7.8 μM).

Product Specifications for JW 67

Molecular Weight

394.38

Formula

C21H18N2O6

Storage

Store at +4°C

Purity

≥97% (HPLC)

Chemical Name

Trispiro[3H-indole-3,2'-[1,3]dioxane-2'',3'''-[3H]indole]-2,2'''(1H,1'''H)-dione

CAS Number

442644-28-2

PubChem ID

644733

InChI Key

BTXRSHKJNDFHGA-UHFFFAOYSA-N

SMILES

O=C1NC6=C(C=CC=C6)C21OCC3(COC(C(NC5=C4C=CC=C5)=O)4OC3)CO2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 39.44 100

Preparing Stock Solutions for JW 67

The following data is based on the product molecular weight 394.38.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.54 mL 12.68 mL 25.36 mL
5 mM 0.51 mL 2.54 mL 5.07 mL
10 mM 0.25 mL 1.27 mL 2.54 mL
50 mM 0.05 mL 0.25 mL 0.51 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for JW 67

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for JW 67

For research use only

Customer Reviews for JW 67 (1)

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  • Induces beta-Catenin degradation
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HepG2, and Huh,
    Verified Customer | Posted 02/10/2023
    When treated in to hepatoma cells (0.5uM) for 48 hours, it substantially induced the degradation of b catenin.
    JW 67 4651

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