Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-48894
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI5D6
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 25-261 of human Kallikrein 2 (RefSeq#: NP_005542, Uniprot#: P20151) produced in SF9 cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free
Western Blot:Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free[NBP3-48894]
Western Blot:Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free[NBP3-48894]
Immunohistochemistry:Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free[NBP3-48894]
Immunohistochemistry:Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free[NBP3-48894]
Western Blot:Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free[NBP3-48894]
Western Blot:Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free[NBP3-48894]![Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free Western Blot;Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free[NBP3-48894]-](https://resources.rndsystems.com/images/products/nbp3-48894_mouse-kallikrein-2-mab-oti5d6-azide-and-bsa-free-western-blot-75202622504313.jpg "Western Blot;Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free[NBP3-48894]-")
Western Blot;Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free[NBP3-48894]-
HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY KLK2 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLK2. Positive lysates![Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free Immunohistochemistry;Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free[NBP3-48894]-](https://resources.rndsystems.com/images/products/nbp3-48894_mouse-kallikrein-2-mab-oti5d6-azide-and-bsa-free-immunohistochemistry-75202622591713.jpg "Immunohistochemistry;Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free[NBP3-48894]-")
Immunohistochemistry;Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free[NBP3-48894]-
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KLK2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.Applications for Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Reconstitution
We recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. For conjugation experiments, we strongly recommend performing another round of desalting process
Formulation
Lyophilized from 1X PBS (pH 7.3)
Format
Azide and BSA Free
Preservative
8% Trehalose
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Calculators
Background: Kallikrein 2
Alternate Names
Klk1c2, KLK2, KLK2A2, Ton
Gene Symbol
KLK2
Additional Kallikrein 2 Products
Product Documents for Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Kallikrein 2 Antibody (OTI5D6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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