KBTBD7 Antibody - BSA Free

Western Blot: KBTBD7 AntibodyBSA Free [NBP3-05059]

Western Blot: KBTBD7 Antibody [NBP3-05059] - Analysis of extracts of various cell lines, using KBTBD7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit

Immunocytochemistry/ Immunofluorescence: KBTBD7 Antibody - BSA Free [NBP3-05059] -

KBTBD7 is highly expressed in NSCLC tissues and cell lines. (A) KBTBD7 expression is positive in NSCLC tissues: squamous carcinoma (i) and adenocarcinoma (ii) but negative in paired normal bronchial (iii) and alveolar epithelial cells (iv). Magnification, ×200. (B) Western blot results show that KBTBD7 is highly expressed in fresh NSCLC tissues (C) compared to that in corresponding noncancerous tissues (N). Relative quantification of protein expression was analyzed by the Image J software. *p < 0.05; **p < 0.01. (C) Western blot results indicate that the KBTBD7 protein level is increased in A549, SK ‐MES ‐1, H460, H292, and H1299 cells compared to that in HBE cells. Relative quantification of protein expression was analyzed by the Image J software. (D) Immunofluorescence assays indicate that KBTBD7 is located in the cytoplasm of NSCLC cell lines. Magnification, ×400. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35499228), licensed under a CC-BY license. Not internally tested by Novus Biologicals.