KRAS Antibody (OTI2C1)

Novus Biologicals | Catalog # NBP2-45536

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2C1
View all KRAS Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human KRAS (NP_203524) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KRAS Antibody (OTI2C1)

Western Blot: KRAS Antibody (OTI2C1) [NBP2-45536]

Western Blot: KRAS Antibody (OTI2C1) [NBP2-45536]

Western Blot: KRAS Antibody (OTI2C1) [NBP2-45536] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Positive lysates LY409579 (100ug) and LC409579 (20ug).
Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536]

Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536]

Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536] - Staining of paraffin-embedded human normal kidney using anti-KRAS clone OTI2C1 mouse monoclonal antibody @ 1:100 with D12-18 Polink1 anti-Ms HRP DAB detection kit. NBP2-45536 tissue screen used heat-induced epitope retrieval buffer ACCEL, pH8.7 in pressure cooker. Results show mostly cytoplasmic staining.
Western Blot: KRAS Antibody (OTI2C1) [NBP2-45536]

Western Blot: KRAS Antibody (OTI2C1) [NBP2-45536]

Western Blot: KRAS Antibody (OTI2C1) [NBP2-45536] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536]

Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536]

Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536] - Staining of human normal brain using anti-KRAS clone OTI2C1 mouse monoclonal antibody @ 1:100 with D12-18 Polink1 anti-Ms HRP DAB detection kit. NBP2-45536 tissue screen used heat-induced epitope retrieval buffer ACCEL, pH8.7 in pressure cooker. Results show nuclear and cytoplasmic staining.
Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536]

Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536]

Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536] - Staining of human normal colon using anti-KRAS clone OTI2C1 mouse monoclonal antibody @ 1:100 with D12-18 Polink1 anti-Ms HRP DAB detection kit. NBP2-45536 tissue screen used heat-induced epitope retrieval buffer ACCEL, pH8.7 in pressure cooker. Results show nuclear and cytoplasmic staining.
Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536]

Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536]

Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536] - Staining of human normal brain using anti-KRAS clone OTI2C1 mouse monoclonal antibody @ 1:100 with D12-18 Polink1 anti-Ms HRP DAB detection kit. NBP2-45536 tissue screen used heat-induced epitope retrieval buffer ACCEL, pH8.7 in pressure cooker. Results show nuclear and cytoplasmic staining.
KRAS Antibody (OTI2C1)

KRAS Antibody (OTI2C1)[NBP2-45536]

KRAS Antibody (OTI2C1)[NBP2-45536]Simple Western� analysis of endogenous protein DCPS from HELA lysates (0.2 mg/mL) using DCPS Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for KRAS Antibody (OTI2C1)

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KRAS

KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Alternate Names

cellular c-Ki-ras2 proto-oncogene, c-Ki-ras, c-K-ras, GTPase KRas, KI-RAS, Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog, K-Ras 2, K-ras p21 protein, KRAS1, K-RAS2A, K-RAS2B, KRAS2NS3, K-RAS4A, K-RAS4B, NS, oncogene KRAS2, PR310 c-K-ras oncogene, RASK2c-Kirsten-ras protein, transforming protein p21, v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Gene Symbol

KRAS

Additional KRAS Products

Product Documents for KRAS Antibody (OTI2C1)

Certificate of Analysis

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Product Specific Notices for KRAS Antibody (OTI2C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KRAS Antibody (OTI2C1)

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Protocols

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