Recombinant E. coli L-Asparaginase His Protein

Name: Recombinant E. coli L-Asparaginase His Protein
Brand: Novus Biologicals
SKU: NBP1-72323
Price: 349 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-72323

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all L-Asparaginase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-338 of E.coli L-Asparaginase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT IHEYTPLMDS SDMTPEDWQH IAEDIKAHYD DYDGFVILHG TDTMAYTASA LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA NYPINEVTLF FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG ELIVHPITPQ PIGVVTIYPG ISADVVRNFL RQPVKALILR SYGVGNAPQN KAFLQELQEA SDRGIVVVNL TQCMSGKVNM GGYATGNALA HAGVIGGADM TVEATLTKLH YLLSQELDTE TIRKAMSQNL RGELTPDD

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

39.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli L-Asparaginase His Protein

Recombinant E. coli L-Asparaginase Protein [NBP1-72323]

Formulation, Preparation, and Storage

NBP1-72323

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: L-Asparaginase

AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB). AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E.coli ansA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

AnsA, AnsB, Asparaginase, Colaspase, Cytoplasmic asparaginase I, L ASNase I, L ASNase II, L asparaginase 1, L asparaginase 2, L asparaginase I, L asparaginase II, L asparaginase II precursor, L asparagine amidohydrolase I, L asparagine amidohydrolase II

Additional L-Asparaginase Products

Product Documents for Recombinant E. coli L-Asparaginase His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant E. coli L-Asparaginase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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