Laurdan

Key Product Details

Product Description

Key information: Laurdan is a polarity sensitive fluorescent probe for imaging lipid rafts in membranes (also known as lipid microdomains). Suitable for live and fixed cells as well as in whole tissues imaging with multiphoton microscopy.

Used for: imaging lipid raft and cell membrane investigations.

Application: fluorescence microscope. Suitable for use with generalized polarization (GP) imaging and scanning fluorescence correlation spectroscopy (FCS).

Properties and Photophysical Data: When Laurdan is incorporated into membranes, its fluorescence spectra is sensitive to the physical state of the surrounding phospholipids. Excitation and emission maxima (λ) are 366 nm and 497 nm, respectively; quantum yield = 0.61; extinction coefficient = 19,500 M^-1cm^-1. In gel and liquid phase membranes, excitation and emission maxima (λ) are 440 nm and 490 nm, respectively.

Optical Data

Product Specifications for Laurdan

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for Laurdan

The following data is based on the product molecular weight 353.55.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

3 1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	14.14 mL	70.71 mL	141.42 mL
1 mM	2.83 mL	14.14 mL	28.28 mL
2 mM	1.41 mL	7.07 mL	14.14 mL
10 mM	0.28 mL	1.41 mL	2.83 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 5 publications citing the usage of this product.

• Owen Quantitative imaging of membrane lipid order in cells and organisms. Nat.Protoc. 2011 PMID: 22157973
• Sanchez Laurdan generalized polarization fluctuations measures membrane packing micro-heterogeneity in vivo. Proc.Natl.Acad.Sci.U.S.A. 2012 PMID: 22529342

Product Documents for Laurdan