Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric)

Name: Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric)
Brand: Novus Biologicals
SKU: KA1329
Price: 559 USD
Availability: InStock

Novus Biologicals | Catalog # KA1329

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Product Documents

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Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Compounds expected to inhibit Lipoxygenases (10 uL/well. All samples should be assayed in duplicate at minimum.)

View all Lipoxygenase Inhibitor Screening Activity Assays »

Product Specifications

Assay Type

Colorimetric

Kit Type

Assay Kit (Colorimetric)

Species

Human

Description

Lipoxygenase Inhibitor Screening Assay Kit is a measurement of detecting hydeoperoxides produced in the lipoxygenation reaction.

Scientific Data Images for Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric)

Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric) [KA1329] - Inhibition of soybean 15-lipoxygenase by NDGA (IC50= 9 uM).

Kit Contents for Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric)

15-Lipoxygenase Standard
96-Well Cover Sheet
96-Well Solid Plate (Colorimetric Assay)
Arachidonic Acid (Substrate)
Developing Reagent 1
Developing Reagent 2
Linoleic Acid (Substrate)
Lipoxygenase Inhibitor Screening Assay Buffer (10X)
Nordihydroguaiaretic Acid (NDGA) Positive Control Inhibitor
Potassium Hydroxide

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Lipoxygenase Inhibitor Screening

Lipoxygenases (LOs) are non-heme iron-containing dioxygenases that catalyze the addition of molecular oxygen to fatty acids containing a cis,cis-1,4-pentadiene system. The initial product of this reaction is a 4-hydroperoxy cis-trans-1,3-conjugated pentadienyl moiety within the unsaturated fatty acid. The three main LO enzymes are designated 5-, 12-, and 15-LO based on the position of the introduced hydroperoxide. Linoleate and arachidonate are the common substrates for LOs in plants and animals.

Additional Lipoxygenase Inhibitor Screening Products

Product Documents for Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric)

Download Product Datasheets

Download 5-Lipoxygenase Assay/Inhibitor Screening Assay Kit

Certificate of Analysis

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Product Specific Notices for Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Citations for Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric)

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