LMP7/PSMB8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30294

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LMP7/PSMB8 Primary Antibodies »

Product Specifications

Immunogen

Between 226 and 276

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LMP7/PSMB8 Antibody - BSA Free

LMP7/PSMB8 Antibody

Immunoprecipitation:LMP7/PSMB8 AntibodyNBP3-30294] -

Immunoprecipitation:LMP7/PSMB8 AntibodyNBP3-30294] -Detection of human PSMB8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PSMB8 antibody NBP3-30294 (lot NBP3-30294-1) used for IP at 6 µg per reaction. PSMB8 was also immunoprecipitated by rabbit anti-PSMB8 antibodies. For blotting immunoprecipitated PSMB8, NBP3-30294 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for LMP7/PSMB8 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 µg/mg lysate

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LMP7/PSMB8

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Long Name

Large Multifunctional Protease 7

Alternate Names

ALDD, D6S216E, JMP, NKJO, Proteasome Component C13, Proteasome Subunit Y2, PSMB5i, PSMB8, RING10

Gene Symbol

PSMB8

Additional LMP7/PSMB8 Products

Product Documents for LMP7/PSMB8 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LMP7/PSMB8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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Protocols

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