LPS from E. Coli, TLR4 ligand

Novus Biologicals | Catalog # NBP2-25295

Novus Biologicals
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Key Product Details

Applications

Functional Assay

Product Summary for LPS from E. Coli, TLR4 ligand

This product is purified by Phenol extraction
Appearance (Color): Clear to semi-yellow liquid
Solubility (Solvent): Water
Solubility (Conc): 4.90 - 5.10 mg/ml
Solubility (Turbidity) :Faint Hazy to Hazy
Solubility (Color) :Colorless to Light Yellow
Protein Content (Method) :Lowry
Prot. Content (% Protein): 3.00 %
Potency (Sample EU/mg): > 500000 EU/mg
Lipopolysaccharide compounds are highly pyrogenic. Avoid inhalation of any LPS and prevent these compounds from entering the bloodstream

Product Specifications

Application Notes

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). Use in Functional reported in scientific literature (PMID: 26121241)

Formulation, Preparation, and Storage

Preparation Method

Reconstitute with 1 mL sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations.

Purification

Ion exchange chromatography

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LPS from E. Coli, TLR4 ligand

Alternate Names

L P S, LPS, LPS from E. Coli, LPS from E. Coli TLR4 ligand, TLR Ligand, TLR4 Ligand

Additional LPS from E. Coli, TLR4 ligand Products

Product Documents for LPS from E. Coli, TLR4 ligand

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for LPS from E. Coli, TLR4 ligand

This product is purified by Phenol extraction
Appearance (Color): Clear to semi-yellow liquid
Solubility (Solvent): Water
Solubility (Conc): 4.90 - 5.10 mg/ml
Solubility (Turbidity) :Faint Hazy to Hazy
Solubility (Color) :Colorless to Light Yellow
Protein Content (Method) :Lowry
Prot. Content (% Protein): 3.00 %
Potency (Sample EU/mg): > 500000 EU/mg
Lipopolysaccharide compounds are highly pyrogenic. Avoid inhalation of any LPS and prevent these compounds from entering the bloodstream

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Citations for LPS from E. Coli, TLR4 ligand

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  • LPS from E. Coli, TLR4 ligand
    Name: Walter Jackson
    Verified Customer | Posted 02/26/2021
    It worked well in the polarization and differentiation of mouse M0 macrophages into M1 macrophages in vitro.

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FAQs for LPS from E. Coli, TLR4 ligand

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  • Q: I want to use flow cytometry to quantify dendritic cells in lung tissue. Do you know the protocol for using LPS NBP2-25295 as a stimulant for dendritic cells? Is it similar to an overnight stimulation of PMA/ionomycin for T cells?

    A: For this product we suggest looking at the publications to see what they did. The publication listed used 10ug/ml and incubated for 12hrs.

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