Lung Matched Tumor and Normal Tissue Lysate
Novus Biologicals | Catalog # NBP2-47091
Key Product Details
Species
Product Summary for Lung Matched Tumor and Normal Tissue Lysate
1 x Human lung normal tissue lysate (matched) (1 mg/ml, 100 ug/vial)
Matched Tumor & Normal Tissue Lysate Set
Diagnosis: AdenocarcinomaSex: Male
Age: 74
Grade: 2
Stage: Not staged
Tumor Pathology Data
Location: Right lower lobe
Gross findings: Tumor size 4 cm diameter, well demarcated. Cut section soft, hemorrhagic and necrotic. Ipsilateral hilar lymph nodes: 0
Microscopic Findings
Tumor shows proliferation of malignant epithelial cell clusters. Epithelial cells form clusters with irregular, basophilic, large nuclei and predominant nucleoli. Nuclear chromatin is coarse and irregularly distributed. Mitotic figures are evident. The surrounding stroma is hemorrhagic and infiltrated by large numbers of lymphocytes, plasma cells, eosinophils and neutrophils. Tumor necrosis and blood vessel invasion are revealed.
Preparation Method
Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify.
Extraction 1: PBS, pH 7.4; 1 ug/ml Aprotinin; 1 mM NaF
Modified RIPA Buffer: 1 mM EDTA; 1 ug/ml Pepstatin-A; 0.1% SDS; 0.25% Na deoxycholate; 1 ug/ml Leupeptin; 1 mM PMSF; 1 mM Na3VO4
Product Specifications
Application Notes
For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use.
Type
Life Stage
Tissue
Formulation, Preparation, and Storage
Concentration
Shipping
Storage
Product Documents for Lung Matched Tumor and Normal Tissue Lysate
Certificate of Analysis
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Product Specific Notices for Lung Matched Tumor and Normal Tissue Lysate
The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.
This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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