LY 294002 hydrochloride
Tocris Bioscience | Catalog # 1130
Key Product Details
Description
Product Description
LY 294002 hydrochloride is a prototypical PI 3-kinase inhibitor (IC50 values are 0.31, 0.73, 1.06 and 6.60 μM for PI 3-Kβ, PI 3-Kα, PI 3-Kδ and PI 3-Kγ respectively). Also inhibits other kinases including, CK2, mTOR, PLK1, PIM1 and PIM3. Inhibits proliferation and induces apoptosis in human colon cancer cells in vitro and in vivo. Suppresses proliferation of mESCs. Inhibits autophagic sequestration in rat hepatocytes.
Negative Control also available.
Product Specifications for LY 294002 hydrochloride
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 25 |
Preparing Stock Solutions for LY 294002 hydrochloride
The following data is based on the product molecular weight 343.81.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.25 mM | 11.63 mL | 58.17 mL | 116.34 mL |
| 1.25 mM | 2.33 mL | 11.63 mL | 23.27 mL |
| 2.5 mM | 1.16 mL | 5.82 mL | 11.63 mL |
| 12.5 mM | 0.23 mL | 1.16 mL | 2.33 mL |
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 105 publications citing the usage of this product.
- Bain The selectivity of protein kinase inhibitors: a further update. Biochem.J. 2007 PMID: 17850214
- Blommaart The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes. Eur.J.Biochem. 1997 PMID: 9030745
- Lianguzova Phosphoinositide 3-kinase inhibitor LY294002 but not serum withdrawal suppresses proliferation of murine embryonic stem cells. Cell Biol.Int. 2007 PMID: 17321171
- Vlahos A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY 294002). J.Biol.Chem. 1994 PMID: 8106507
Product Documents for LY 294002 hydrochloride
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LY 294002 hydrochloride
For research use only
Citations for LY 294002 hydrochloride
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Customer Reviews for LY 294002 hydrochloride (7)
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Species: HumanAssay Type: In VitroCell Line/Tissue: AGSVerified Customer | Posted 09/28/2024Aliquot the reconstituted compound to avoid freezing and thawing repeatedly.To understand the involvement of the PI3K/Akt pathway in the regulation of Snail activation by Helicobacter pylori incubation in gastric cancer cells AGS.
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Species: HumanAssay Type: In VitroVerified Customer | Posted 03/15/2023This product works as expected and is easy to manage.We use this product for in vitro cell culture assays.
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Species: MouseAssay Type: In VitroCell Line/Tissue: BV2 microglia cellsVerified Customer | Posted 06/18/2019BV2 microglia cells were incubated for one hour with 10µM LY294002 prior to treatment with 1µM LPA. Western blot analysis revealed a possible role for PI3K in the LPA induced expression of pAMPK.
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Species: HumanAssay Type: In VitroCell Line/Tissue: OsteosarcomaVerified Customer | Posted 12/13/2018Human osteosarcoma cells were incubated with 10 μM LY 294002 for 30 min prior to treatment with 10 μM LPA to estimate COX-2 expression using Western blot analysis. LY 294002 abolished LPA-induced COX-2 expression.
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Species: HumanAssay Type: In VitroCell Line/Tissue: MG-63 osteosarcoma cellsVerified Customer | Posted 10/15/2018MG-63 cells were incubated with PD169316 (25 μM), LY294002 (10 μM) or Akt-1/2 (5 μM Akt-I) for 30 min prior to 15d-PGJ2 treatment (20 μM) for 1 h to follow pAkt expression using Western blot. Akt-1/2 completely blocked 15d-PGJ2-induced Akt phosphorylation in MG-63 cells.
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Species: HumanAssay Type: In VitroCell Line/Tissue: CHO K1Verified Customer | Posted 01/09/2018I used LY294002 hydrochloride 10µM to inhibit pAKT response of FFAR GPCR activators (15min 37°C).
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Species: MouseAssay Type: In VitroCell Line/Tissue: Ovarian theca cellsVerified Customer | Posted 01/04/2018Mouse ovarian theca cells isolated from 60-day old mice were used. Cultured Ptenfl/fl and tPtenMT ovarian theca cells were pretreated with vehicle (DMSO), LY294002 (50 mM), or API-2 (7.5 mM) for 1 h before the addition of LH (50 ng/mL) for 30 min. Western blotting was used to detect Foxo1 phosphorylation in cultured ovarian theca cells.
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