Lysozyme Conjugation Substrate, Biotin

Name: Lysozyme Conjugation Substrate, Biotin
Brand: R&D Systems
SKU: SP-100

R&D Systems | Catalog # SP-100

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Product Documents

Discontinued Product

SP-100 has been discontinued. View all Lysozyme products.

Preparation & Storage
Calculators
Background
Product Documents
Specific Notices

Key Product Details

Species

Chicken

Applications

Enzyme Activity

Conjugate

Biotin

Formulation, Preparation, and Storage

Formulation

Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.

Reconstitution Instructions

Reconstitute in 70 μl of sterile, deionized water to generate a 0.7 mg/ml (50 μM) stock solution.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Lysozyme

Lysozyme has been used extensively as a substrate to study the Ubiquitin-dependent proteolytic pathway in vitro. This protein is an N-end rule substrate, since the recognition of protein requires the binding of an E3 ligase to the N-terminal lysine residue. Ubiquitin-lysozyme conjugates can be formed in the presence of ATP, Ubiquitin and the appropriate conjugation enzyme source(s). This substrate protein can be used as a positive control with crude fractions (F-340, F-360, F-370, F-372) and conjugation kits (K-915, K-930,
K-935, K-960). Additionally, this lysozyme is labeled with biotin for use with avidin affinity/detection reagents.

Alternate Names

EC 3.2.1.17, lysozyme, lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C, lysozyme C, LZM, renal amyloidosis

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Product Documents for Lysozyme Conjugation Substrate, Biotin

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Certificate of Analysis

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Product Specific Notices for Lysozyme Conjugation Substrate, Biotin

For research use only

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