Recombinant Human MafK His Protein

Novus Biologicals | Catalog # NBP2-22942

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all MafK Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-156 of Human MafK

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTTNPKPNKA LKVKKEAGEN APVLSDDELV SMSVRELNQH LRGLTKEEVT RLKQRRRTLK NRGYAASCRI KRVTQKEELE RQRVELQQEV EKLARENSSM RLELDALRSK YEALQTFART VARGPVAPSK VATTSVITIV KSTELSSTSV PFSAAS

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MafK His Protein

SDS-PAGE: Recombinant Human MafK His Protein [NBP2-22942]

SDS-PAGE: Recombinant Human MafK His Protein [NBP2-22942]

SDS-Page: Recombinant Human MafK Protein [NBP2-22942]

Formulation, Preparation, and Storage

NBP2-22942
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MafK

MAFK belongs to the bZIP family and Maf subfamily. Members of the Maf family of basic region/leucine zipper (bZIP) transcription factors affect transcription in either a positive or negative fashion, depending on their particular protein partner and the context of the target promoter. Small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Recombinant human MAFK protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog K

Alternate Names

NFE2U

Gene Symbol

MAFK

Additional MafK Products

Product Documents for Recombinant Human MafK His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human MafK His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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