MAML3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86674

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MAML3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GVGMVSGFGQSMLVNSAITQQHPQMKGPVGQALPRPQAPPRLQSLMGTVQQGAQSWQQRSLQGMPGRTSGELGPFNNGASYPLQAGQPR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAML3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MAML3 Antibody [NBP1-86674]

Immunocytochemistry/ Immunofluorescence: MAML3 Antibody [NBP1-86674]

Immunocytochemistry/Immunofluorescence: MAML3 Antibody [NBP1-86674] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674]

Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674]

Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674] - Analysis in human endometrium and skeletal muscle tissues using NBP1-86674 antibody. Corresponding MAML3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674]

Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674]

Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674]

Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674]

Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674] - Staining of human Endometrium shows moderate nuclear positivity in stromal and glandular cells.
Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674]

Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674]

Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674] - Staining of human Cervix shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674]

Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674]

Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674] - Staining of human Lymph node shows moderate nuclear positivity in germinal center and non-germinal center cells.
Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674]

Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674]

Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.

Applications for MAML3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAML3

There are 3 MAML (mastermind-like) proteins in mammals (MAML1-3). MAML3 is a coactivator for NOTCH signaling. MAML3 associates with NOTCH and CSL in a ternary complex and functions to activate transcription of HES and hairy-related basic helix-loop-helix transcription factors.

Alternate Names

CAG/CTG 3, ERDA3, MAM-2, mam-3, mastermind-like 3 (Drosophila), mastermind-like protein 3, polyglutamine rich, trinucleotide repeat containing 3

Gene Symbol

MAML3

Additional MAML3 Products

Product Documents for MAML3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MAML3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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