Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate

R&D Systems | Catalog # ES004

Substrate for BACE-1
R&D Systems
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Key Product Details

Conjugate

7-Methoxycoumarin

Applications

Bioactivity
View all Protease Substrates Fluorescent Probes and Dyes »

Product Specifications

Source


Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-NH2
Mca: (7-Methoxycoumarin-4-yl) acetyl, Dnp: 2, 4-Dinitrophenyl

Predicted Molecular Mass

2001 Da

Activity

The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence.

The substrate contains the Swedish mutation of the amyloid precursor protein (APP) beta -secretase cleavage site. A K-R-R sequence is added for easy solubilization. It can be used to measure the activity of BACE-1 (R&D Systems, Catalog # 931-AS) and thimet oligopeptidase (Koike, H. et al., 1999, J. Biochem. 126:235).

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Formulation, Preparation, and Storage

ES004
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light

Product Documents for Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate

Certificate of Analysis

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Product Specific Notices for Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate

For research use only

Citations for Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate

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