Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate
Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate Summary
Mca: (7-Methoxycoumarin-4-yl) acetyl, Dnp: 2, 4-Dinitrophenyl
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a stock solution in dimethyl sulfoxide (DMSO) (100 μL) at a concentration of 10.0 mg/mL or 4.1 mM..|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light|
Citations for Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Evaluation of an Allosteric BACE inhibitor peptide to identify mimetics that can interact with the Loop F Region of the enzyme and prevent APP cleavage
Authors: J Campagna, K Vadivel, B Jagodzinsk, M Jun, T Bilousova, P Spilman, V John
J. Mol. Biol., 2018;0(0):.
Sample Types: Recombinant Protein
Gleevec shifts APP processing from a ?-cleavage to a nonamyloidogenic cleavage
Authors: WJ Netzer, K Bettayeb, SC Sinha, M Flajolet, P Greengard, V Bustos
Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
Sample Types: Protein
Applications: Enzyme Assay
Localization and Processing ofï¿½theï¿½Amyloid-? Protein Precursor inï¿½Mitochondria-Associated Membranes
Authors: Dolores Del Prete
J. Alzheimers Dis, 2016;0(0):.
Inhibition of amyloid beta-peptide production by blockage of beta-secretase cleavage site of amyloid precursor protein.
Authors: Na CH, Jeon SH, Zhang G, Olson GL, Chae CB
J. Neurochem., 2007;101(6):1583-95.
Sample Types: Recombinant Protein
BACE1- and BACE2-expressing human cells: characterization of beta-amyloid precursor protein-derived catabolites, design of a novel fluorimetric assay, and identification of new in vitro inhibitors.
Authors: Andrau D, Dumanchin-Njock C, Ayral E, Vizzavona J, Farzan M, Boisbrun M, Fulcrand P, Hernandez JF, Martinez J, Lefranc-Jullien S, Checler F
J. Biol. Chem., 2003;278(28):25859-66.
Sample Types: Cell Lysates
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