MCM10 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92102

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MCM10 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QTTLSNLVVKGTNLIIQETRQKLGIPQKSLSCSEEFKELMDLPTCGARNLKQHLAKATASGIMGSPKPAIKSISASALLKQQKQRMLEMRRRKSEEIQK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM10 Antibody - BSA Free

Western Blot: MCM10 Antibody [NBP1-92102]

Western Blot: MCM10 Antibody [NBP1-92102]

Western Blot: MCM10 Antibody [NBP1-92102] - Analysis in control (vector only transfected HEK293T lysate) and MCM10 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102]

Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102]

Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102]

Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102]

Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102]

Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102]

Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102]

Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102]

Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102] - Staining of human lymph node shows strong nuclear positivity in a subset of non-germinal center cells.
Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102]

Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102]

Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102] - Staining of human small intestine shows strong positivity in lymphoid cells.

Applications for MCM10 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCM10

MCM10 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

Alternate Names

CNA43homolog of yeast MCM10, DNA43, hsMCM10, MCM10 minichromosome maintenance deficient 10, MCM10 minichromosome maintenance deficient 10 (S. cerevisiae), MGC126776, minichromosome maintenance complex component 10, PRO2249, protein MCM10 homolog

Gene Symbol

MCM10

Additional MCM10 Products

Product Documents for MCM10 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MCM10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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