Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MCM4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 750 and 800 of human Minichromosome Maintenance 4 using the numbering given in entry NP_005905.2 (GeneID 4173).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MCM4 Antibody - BSA Free

Western Blot: MCM4 Antibody [NB100-290]

Western Blot: MCM4 Antibody [NB100-290]

Western Blot: MCM4 Antibody [NB100-290] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.
Immunohistochemistry: MCM4 Antibody [NB100-290]

Immunohistochemistry: MCM4 Antibody [NB100-290]

Immunohistochemistry: MCM4 Antibody [NB100-290] - Sample: FFPE section of human testicular seminoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-MCM4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for MCM4 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MCM4

The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM4 is part of the core MCM complex that includes MCM4, MCM6, and MCM7. The core complex possesses DNA helicase activity. MCM4 is phosphorylated by CDC2 kinase resulting in a reduction of helicase activity and chromatin binding. Alternate names for MCM4 include DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21, CDC54, hCdc21, and MGC33310.

Long Name

minichromosome maintenance complex component 4

Alternate Names

CDC21, CDC54, hCdc21, IMD54, NKCD, NKGCD, P1-CDC21

Entrez Gene IDs

4173 (Human)

Gene Symbol

MCM4

UniProt

P33991 (Human)

Additional MCM4 Products

Product Documents for MCM4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MCM4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MCM4 Antibody - BSA Free

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Protocols

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FAQs

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