MEST Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87299

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MEST Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HSWKSSGKFFTYKGLRIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHHYSIFEQASIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPL

Reactivity Notes

Rat (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEST Antibody - BSA Free

Western Blot: MEST Antibody [NBP1-87299]

Western Blot: MEST Antibody [NBP1-87299]

Western Blot: MEST Antibody [NBP1-87299] - Analysis in control (vector only transfected HEK293T lysate) and MEST over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419353).
Immunocytochemistry/ Immunofluorescence: MEST Antibody [NBP1-87299]

Immunocytochemistry/ Immunofluorescence: MEST Antibody [NBP1-87299]

Immunocytochemistry/Immunofluorescence: MEST Antibody [NBP1-87299] - Immunofluorescent staining of human cell line A-431 shows positivity in golgi apparatus. Antibody staining in green.

Applications for MEST Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEST

MEST encodes a member of the [alpha]/[beta] hydrolase fold family and has isoform specific imprinting. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Three transcript variants encoding two distinct isoforms have been identified for this gene. A pseudogene for this locus is located on chromosome 6. [provided by RefSeq]

Alternate Names

DKFZp686L18234, EC 3.-, mesoderm specific transcript (mouse) homolog, mesoderm specific transcript homolog (mouse), mesoderm-specific transcript homolog protein, MGC111102, MGC8703, paternally expressed gene 1, PEG1Paternally-expressed gene 1 protein

Gene Symbol

MEST

Additional MEST Products

Product Documents for MEST Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MEST Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MEST Antibody - BSA Free

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Protocols

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FAQs

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