Catalog Number: 1230
Chemical Name: N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid
Biological Activity
Cytotoxic agent. Inhibits thymidylate synthetase and de novo purine synthesis. Potent folic acid antagonist; inhibits dihydrofolate reductase. Also inhibits Ras carboxyl methylation in DKOB8 cells, leading to decreased p44 and Akt activation.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 100 mM in DMSO
  • Purity:
  • Storage:
    Store at RT
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Evidence for direct inhibition of de novo protein synthesis in human MCF-7 breast cells as a principal mode of metabolic inhibition by methotrexate.
    Allegra et al.
    J.Biol.Chem., 1987;262:13520
  2. Mechanism of thymidylate synthase inhibition by methotrexate in human neoplastic cell lines and normal human myeloid progenitor cells.
    Chu et al.
    J.Biol.Chem., 1990;265:8470
  3. The pharmacology and clinical use of methotrexate.
    Jolivet et al.
    N.Engl.J.Med., 1983;309:1094
  4. Targeting Ras signaling through inhibition of carboxyl methylation: an unexpected property of methotrexate.
    Winter-Vann et al.
    Proc.Natl.Acad.Sci.U.S.A., 2003;100:6529

The citations listed below are publications that use Tocris products. Selected citations for Methotrexate include:

Showing Results 1 - 1 of 1

  1. RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs.
    Authors: Robinson Et al.
    PLoS One
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