Cytotoxic agent. Inhibits thymidylate synthetase and de novo purine synthesis. Potent folic acid antagonist; inhibits dihydrofolate reductase. Also inhibits Ras carboxyl methylation in DKOB8 cells, leading to decreased p44 and Akt activation.
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Evidence for direct inhibition of de novo protein synthesis in human MCF-7 breast cells as a principal mode of metabolic inhibition by methotrexate.
Allegra et al.
Mechanism of thymidylate synthase inhibition by methotrexate in human neoplastic cell lines and normal human myeloid progenitor cells.
Chu et al.
The pharmacology and clinical use of methotrexate.
Jolivet et al.
Targeting Ras signaling through inhibition of carboxyl methylation: an unexpected property of methotrexate.
Winter-Vann et al.
The citations listed below are publications that use Tocris products. Selected citations for Methotrexate include:
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