Chemical Name: N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid
Biological ActivityMethotrexate is a cytotoxic agent. Inhibits thymidylate synthetase and de novo purine synthesis. Potent folic acid antagonist; inhibits dihydrofolate reductase. Also inhibits Ras carboxyl methylation in DKOB8 cells, leading to decreased p44 and Akt activation.
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Tocris products are intended for laboratory research use only, unless stated otherwise.
Evidence for direct inhibition of de novo protein synthesis in human MCF-7 breast cells as a principal mode of metabolic inhibition by methotr.
Allegra et al.
Mechanism of thymidylate synthase inhibition by methotr. in human neoplastic cell lines and normal human myeloid progenitor cells.
Chu et al.
The pharmacology and clinical use of methotr.
Jolivet et al.
Targeting Ras signaling through inhibition of carboxyl methylation: an unexpected property of methotr.
Winter-Vann et al.
Citations for Methotrexate
The citations listed below are publications that use Tocris products. Selected citations for Methotrexate include:
2 Citations: Showing 1 - 2
Comparison of cell-based assays to quantify treatment effects of anticancer drugs identifies a new application for Bodipy-L-cystine to measure apoptosis.
Authors: Kumar Et al.
Sci Rep 2018;8:16363
RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs.
Authors: Robinson Et al.
PLoS One 2013;8:e78641
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