Methylene Blue
Tocris Bioscience | Catalog # 3213
Key Product Details
Description
Product Description
Methylene Blue is a biological stain and redox indicator. Methylene blue inhibits monoamine oxidase A, tau filament formation and nitric oxide synthase (IC50 values = 0.07 μM, 1.9 μM and 5.3 μM, respectively), and inhibits guanylate cyclase. Methylene Blue delays senescence and stimulates proliferation of fibroblasts and skin cells. In vitro, Methylene Blue promotes wound healing, upregulates elastin expression and production, increases synthesis of heme, is neuroprotective (EC50 = 0.18 nM) and rescues the loss of dopaminergic neurons induced by Rotenone (Cat. No. 3616). Also antimalarial.
Product Specifications for Methylene Blue
Molecular Weight
Formula
Storage
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 3.2 | 10 | |
| DMSO | 3.2 | 10 |
Preparing Stock Solutions for Methylene Blue
The following data is based on the product molecular weight 319.85.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 31.26 mL | 156.32 mL | 312.65 mL |
| 0.5 mM | 6.25 mL | 31.26 mL | 62.53 mL |
| 1 mM | 3.13 mL | 15.63 mL | 31.26 mL |
| 5 mM | 0.63 mL | 3.13 mL | 6.25 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Poteet Neuroprotective actions of methylene blue and its derivatives. PLoS One 2012 PMID: 23118969
- Wen Alternative mitochondrial electron transfer as a novel strategy for neuroprotection. J.Biol.Chem. 2011 PMID: 21454572
- Mayer Inhibition of nitric oxide synthesis by methylene blue. Biochem.Pharmacol. 1993 PMID: 7679577
- Atamna Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways. FASEB J. 2008 PMID: 17928358
- Wischik Selective inhibition of Alzheimer disease-like tau aggregation by phenothiazines. Proc.Natl.Acad.Sci.U.S.A. 1996 PMID: 8855335
- Taniguchi Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins. J.Biol.Chem. 2005 PMID: 15611092
- Hwang Comparison of two soluble guanylyl cyclase inhibitors, methylene blue and ODQ, on sodium nitroprusside-induced relaxation in guinea-pig trachea. Br.J.Pharmacol. 1998 PMID: 9863642
Product Documents for Methylene Blue
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Methylene Blue
For research use only
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Species: HumanVerified Customer | Posted 11/13/2018Methylene blue was used to prepare Eosin Methylene Blue (EMB) agar plate. Excellent product.
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