Rotenone

Tocris Bioscience | Catalog # 3616

Inhibits complex I of the mitochondrial electron transport chain
Tocris Bioscience

Key Product Details

Description

Inhibits complex I of the mitochondrial electron transport chain
View all Oxidative Phosphorylation Inhibitors »

Product Description

Rotenone is a mitochondrial electron transport chain inhibitor (IC50 = 1.7 - 2.2 μM at complex I). Inhibits NADH oxidation by cardiac sarcoplasmic reticulum (IC50 = 3.4 nM). Commonly used pesticide and induces Parkinsonism in animal models. May also inhibit autophagy induction; blocks lysosomal degradation of autophagic vacuoles. Cell-permeable and brain penetrant.

Product Specifications for Rotenone

Molecular Weight

394.42

Formula

C23H22O6

Storage

Store at RT

Purity

≥95% (HPLC)

Chemical Name

(2R,6aS,12aS)-1,2,12,12a-Tetrahydro-8,9-dimethoxy-2-(1-methylethenyl)-[1]benzopyrano[3,4-b]furo[2,3-h][1]benzopyran-6(6aH)-one

CAS Number

83-79-4

PubChem ID

6758

InChI Key

JUVIOZPCNVVQFO-HBGVWJBISA-N

SMILES

C=[C@](C)[C@@H](C5)OC4=C5C(O3)=C(C=C4)C([C@@]([C@@]3([H])CO2)([H])C1=C2C=C(OC)C(OC)=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 39.44 100

Preparing Stock Solutions for Rotenone

The following data is based on the product molecular weight 394.42.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.54 mL 12.68 mL 25.35 mL
5 mM 0.51 mL 2.54 mL 5.07 mL
10 mM 0.25 mL 1.27 mL 2.54 mL
50 mM 0.05 mL 0.25 mL 0.51 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 49 publications citing the usage of this product.

Product Documents for Rotenone

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rotenone

For research use only

Customer Reviews for Rotenone (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Rotenone test for lactylation
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: 293T
    Verified Customer | Posted 02/17/2025
    Rotenone at 10 mM was treated 293t cells for 24 hours
    Rotenone 3616
  • Strong inhibitor of the mitochondrial complex I.
    Name: Anonymous
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: motor neurons
    Verified Customer | Posted 11/12/2021
    Effect of rotenone (10 micromolar) on AMPA induced ROS formation in motor neurons.
    Rotenone 3616
  • Good choice!
    Name: Anton Mukhamedshin
    Species: Human
    Assay Type: Ex Vivo
    Cell Line/Tissue: Platelets
    Verified Customer | Posted 02/22/2019
    Nice delivery time and all works well!
    I use it to inhibit mitochondrial electron transport chain in platelets. It helps to understand the role of mitochondria in thrombosis.

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