Chemical Name: N-[(Phenylmethoxy)carbonyl]-L-leucyl-N-[(1S)-1-formyl-3-methylbutyl]-L-leucinamide
Biological ActivityPotent cell-permeable inhibitor of proteasome (IC50 = 100 nM) and calpain (IC50 = 1.2 μM). Inhibits TNF-α-induced NF-κB activation and IκBα degradation. Induces neurite outgrowth in PC12 cells and has anticancer properties in vitro.
The technical data provided above is for guidance only.
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Tocris products are intended for laboratory research use only, unless stated otherwise.
Potential of the proteasome inhibitor MG-132 as an anticancer agent, alone and in combination.
Banerjee and Liefshitz
Anticancer Res., 2001;21:3941
The ubiquitin-proteasome pathway is required for processing the NF-κB1 precursor protein and the activation of NF-κB.
Palombella et al.
Differential inhibition of calpain and proteasome activities by peptidyl aldehydes of di-Leucine and tri-Leucine.
Tsubuki et al.
Citations for MG 132
The citations listed below are publications that use Tocris products. Selected citations for MG 132 include:
57 Citations: Showing 1 - 10
MARK2/Par1b kinase present at centrosomes and retraction fibres corrects spindle off-centring induced by actin disassembly.
Authors: Hart Et al.
Open Biol 2019;9:180263
Miro proteins prime mitochondria for Parkin translocation and mitophagy.
Authors: Safiulina Et al.
EMBO J 2019;38
Lethal Poisoning of Cancer Cells by Respiratory Chain Inhibition plus Dimethyl α-Ketoglutarate.
Authors: Sica Et al.
Cell Rep 2019;27:820
Co-targeting RNA Polymerases IV and V Promotes Efficient De Novo DNA Methylation in Arabidopsis.
Authors: Gallego-Bartolomé Et al.
Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production.
Authors: Scrivo Et al.
Nat Commun 2019;10:780
Post-translational regulation of lipogenesis via AMPK-dependent phosphorylation of ins.-induced gene.
Authors: Han Et al.
Nat Commun 2019;10:623
Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation.
Authors: Li Et al.
Nat Commun 2018;9:1420
27-Hydroxycholesterol increases α-synuclein protein levels through proteasomal inhibition in human dopaminergic neurons.
Authors: Schommer Et al.
BMC Neurosci 2018;19:17
Loss of the novel Vcp (valosin containing protein) interactor Washc4 interferes with autophagy-mediated proteostasis in striated muscle and leads to myopathy in vivo.
Authors: Kustermann Et al.
Capsaicin-induced TRIB3 upregulation promotes apoptosis in cancer cells.
Authors: Lin Et al.
Cancer Manag Res 2018;10:4237
TRAF3IP2 mediates high glucose-induced endothelin-1 production as well as endothelin-1-induced inflammation in endothelial cells.
Authors: Padilla Et al.
Am J Physiol Heart Circ Physiol 2017;:ajpheart.00478.2017
Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells.
Nat Commun 2017;8(1):150
Ubiquitin ligase SYVN1/HRD1 facilitates degradation of the SERPINA1 Z variant/α-1-antitrypsin Z variant via SQSTM1/p62-dependent selective autophagy.
Authors: Feng Et al.
Noncanonical SQSTM1/p62-Nrf2 pathway activation mediates proteasome inhibitor resistance in multiple myeloma cells via redox, metabolic and translational reprogramming.
Authors: Riz Et al.
Analogs of the novel phytohormone, strigolactone, trigger apoptosis and synergize with PARP inhibitors by inducing DNA damage and inhibiting DNA repair.
Authors: Croglio Et al.
Atrogin-1 Deficiency Leads to Myopathy and Heart Failure in Zebrafish.
Authors: Bühler Et al.
Int J Mol Sci 2016;17
Glycogen Synthase Kinase-3β and Caspase-2 Mediate Ceramide- and Etoposide-Induced Apoptosis by Regulating the Lysosomal-Mitochondrial Axis.
Authors: Lin Et al.
J Cell Sci 2016;11:e0145460
A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly.
Authors: Barr and Bakal
J Neurosci 2015;5:10564
A proteomic study of mitotic phase-specific interactors of EB1 reveals a role for SXIP-mediated protein interactions in anaphase onset.
Authors: Tamura Et al.
Biol Open 2015;4:155
Estrogen receptor alpha mediates epithelial to mesenchymal transition, expression of specific matrix effectors and functional properties of breast cancer cells.
Authors: Bouris Et al.
Matrix Biol 2015;43:42
Extracellular glutamate exposure facilitates group I mGluR-mediated epileptogenesis in the hippocampus.
Authors: Zhao Et al.
Respir Res 2015;35:308
The E3 ubiquitin ligase ARIH1 protects against genotoxic stress by initiating a 4EHP-mediated mRNA translation arrest.
Authors: Stechow Et al.
PLoS One 2015;35:1254
Decreased expression of the NF-κB family member RelB in lung fibroblasts from Smokers with and without COPD potentiates cigarette smoke-induced COX-2 expression.
Authors: Sheridan Et al.
Mol Cell Biol 2015;16:54
SILAC-Based Mass Spectrometry Analysis Reveals That Epibrassinolide Induces Apoptosis via Activating Endoplasmic Reticulum Stress in Prostate Cancer Cells.
Authors: Obakan Et al.
PLoS One 2015;10:e0135788
Up-regulation of TREK-2 potassium channels in cultured astrocytes requires de novo protein synthesis: relevance to localization of TREK-2 channels in astrocytes after transient cerebral ischemia.
Authors: Rivera-Pagán Et al.
PLoS One 2015;10:e0125195
Translational initiation regulated by ATM in dendritic cells development.
Cell Death Dis 2014;5:e1418
Single nucleotide polymorphism-specific regulation of matrix metalloproteinase-9 by multiple miRNAs targeting the coding exon.
Authors: Duellman Et al.
Nucleic Acids Res 2014;42:5518
HSF1 deficiency and impaired HSP90-dependent protein folding are hallmarks of aneuploid human cells.
Authors: Donnelly Et al.
EMBO J 2014;33:2374
Methyllysine reader plant homeodomain (PHD) finger protein 20-like 1 (PHF20L1) antagonizes DNA (cytosine-5) methyltransferase 1 (DNMT1) proteasomal degradation.
Authors: Estève Et al.
J Biol Chem 2014;289:8277
Phosphorylation and degradation of tomosyn-2 de-represses Ins secretion.
Authors: Bhatnagar Et al.
J Biol Chem 2014;289:25276
Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex.
Authors: Liang Et al.
Cell Cycle 2014;211:2249
Mammalian target of rapamycin complex 2 modulates αβTCR processing and surface expression during thymocyte development.
Authors: Chou Et al.
J Immunol 2014;193:1162
Halofuginone-induced amino acid starvation regulates Stat3-dependent Th17 effector function and reduces established autoimmune inflammation.
Authors: Carlson Et al.
J Immunol 2014;192:2167
BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria.
Authors: Choubey Et al.
Arkadia regulates tumor metastasis by modulation of the TGF-β pathway.
Authors: Briones-Orta Et al.
PLoS One 2013;73:1800
A critical role for protein degradation in the nucleus accumbens core in cocaine reward memory.
Authors: Ren Et al.
Proteasomal degradation of eukaryotic elongation factor-2 kinase (EF2K) is regulated by cAMP-PKA signaling and the SCFβTRCP ubiquitin E3 ligase.
Authors: Wiseman Et al.
J Biol Chem 2013;288:17803
Drosophila SOCS36E negatively regulates JAK/STAT pathway signaling via two separable mechanisms.
Authors: Stec Et al.
Mol Biol Cell 2013;24:3000
Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK.
Authors: Shrestha and Draviam
Curr Biol 2013;23:1514
Human bocavirus VP2 upregulates IFN-β pathway by inhibiting ring finger protein 125-mediated ubiquitination of retinoic acid-inducible gene-I.
Authors: Luo Et al.
J Immunol 2013;191:660
Autophagy-mediated turnover of dynamin-related protein 1.
Authors: Purnell and Fox
BMC Neurosci 2013;14:86
Automated tracking of mitotic spindle pole positions shows that LGN is required for spindle rotation but not orientation maintenance.
Authors: Corrigan Et al.
Eur J Neurosci 2013;12:2643
Spatiotemporal organization of Aurora-B by APC/CCdh1 after mitosis coordinates cell spreading through FHOD1.
Authors: Floyd Et al.
J Appl Physiol (1985) 2013;126:2845
Molecular mechanism of mutant p53 stabilization: the role of HSP70 and MDM2.
Authors: Wiech Et al.
PLoS One 2012;7:e51426
On the mechanism of synaptic depression induced by CaMKIIN, an endogenous inhibitor of CaMKII.
Authors: Gouet Et al.
PLoS One 2012;7:e49293
Tgf-β induced Erk phosphorylation of smad linker region regulates smad signaling.
Authors: Hough Et al.
PLoS One 2012;7:e42513
Free fatty acid palmitate impairs the vitality and function of cultured human bladder smooth muscle cells.
Authors: Oberbach Et al.
Proc Natl Acad Sci U S A 2012;7:e41026
Reversible modification of adenomatous polyposis coli (APC) with K63-linked polyubiquitin regulates the assembly and activity of the β-catenin destruction complex.
Authors: Tran and Polakis
J Biol Chem 2012;287:28552
Punctuated cyclin synthesis drives early embryonic cell cycle oscillations.
Mol Biol Cell 2012;23:284
Quantitative profiling of caspase-cleaved substrates reveals different drug-induced and cell-type patterns in apoptosis.
Authors: Shimbo Et al.
Stem Cells 2012;109:12432
Macrophage migration inhibitory factor promotes cell death and aggravates neurologic deficits after experimental stroke.
Authors: Inácio Et al.
J Cereb Blood Flow Metab 2011;31:1093
Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors.
Authors: Bassett Et al.
Sci Rep 2010;190:177
N-MthD.-aspartate receptor- and metabotropic glutamate receptor-dependent long-term depression are differentially regulated by the ubiquitin-proteasome system.
Authors: Citri Et al.
Cancer Res 2009;30:1443
Leptin enhances synthesis of proinflammatory mediators in human osteoarthritic cartilage--mediator role of NO in leptin-induced PGE2, IL-6, and IL-8 production.
Authors: Vuolteenaho Et al.
Mediators Inflamm 2009;2009:345838
The effect of denervation on protein synthesis and degradation in adult rat diaphragm muscle.
Authors: Argadine Et al.
J Exp Med 2009;107:438
Calcineurin-mediated dephosphorylation of c-Jun Ser-243 is required for c-Jun protein stability and cell transformation.
Authors: Huang Et al.
Effects of levo- and dextrosimendan on NF-kappaB-mediated transcription, iNOS expression and NO production in response to inflammatory stimuli.
Authors: Sareila Et al.
Br J Pharmacol 2008;155:884
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Reviews for MG 132
Average Rating: 5 (Based on 1 Review)
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It works well and dissolves perfectly in DMSO. I treated the cells with MG 132 (final concentration: 10 micromolar) in serum free medium for 24 hours.