Catalog Number: 1748
Alternate Names: Z-LLL-al, Z-Leu-Leu-Leu-CHO
Chemical Name: N-[(Phenylmethoxy)carbonyl]-L-leucyl-N-[(1S)-1-formyl-3-methylbutyl]-L-leucinamide
Biological Activity
Potent cell-permeable inhibitor of proteasome (IC50 = 100 nM) and calpain (IC50 = 1.2 μM). Inhibits TNF-α-induced NF-κB activation and IκBα degradation. Induces neurite outgrowth in PC12 cells and has anticancer properties in vitro.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 100 mM in ethanol and to 100 mM in DMSO
  • Purity:
  • Storage:
    Store at -20°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Potential of the proteasome inhibitor MG-132 as an anticancer agent, alone and in combination.
    Banerjee and Liefshitz
    Anticancer Res., 2001;21:3941
  2. The ubiquitin-proteasome pathway is required for processing the NF-κB1 precursor protein and the activation of NF-κB.
    Palombella et al.
    Cell, 1994;78:773
  3. Differential inhibition of calpain and proteasome activities by peptidyl aldehydes of di-Leucine and tri-Leucine.
    Tsubuki et al.
    J.Biochem., 1996;119:572

The citations listed below are publications that use Tocris products. Selected citations for MG 132 include:

Showing Results 1 - 10 of 33

  1. Glycogen Synthase Kinase-3β and Caspase-2 Mediate Ceramide- and Etoposide-Induced Apoptosis by Regulating the Lysosomal-Mitochondrial Axis.
    Authors: Lin Et al.
    J Cell Sci
  2. A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly.
    Authors: Barr and Bakal
    J Neurosci
  3. A proteomic study of mitotic phase-specific interactors of EB1 reveals a role for SXIP-mediated protein interactions in anaphase onset.
    Authors: Tamura Et al.
    Biol Open
  4. Extracellular glutamate exposure facilitates group I mGluR-mediated epileptogenesis in the hippocampus.
    Authors: Zhao Et al.
    Respir Res
  5. The E3 ubiquitin ligase ARIH1 protects against genotoxic stress by initiating a 4EHP-mediated mRNA translation arrest.
    Authors: Stechow Et al.
    PLoS One
  6. Decreased expression of the NF-κB family member RelB in lung fibroblasts from Smokers with and without COPD potentiates cigarette smoke-induced COX-2 expression.
    Authors: Sheridan Et al.
    Mol Cell Biol
  7. Up-regulation of TREK-2 potassium channels in cultured astrocytes requires de novo protein synthesis: relevance to localization of TREK-2 channels in astrocytes after transient cerebral ischemia.
    Authors: Rivera-Pagán Et al.
    PLoS One
  8. Single nucleotide polymorphism-specific regulation of matrix metalloproteinase-9 by multiple miRNAs targeting the coding exon.
    Authors: Duellman Et al.
    Nucleic Acids Res
  9. Methyllysine reader plant homeodomain (PHD) finger protein 20-like 1 (PHF20L1) antagonizes DNA (cytosine-5) methyltransferase 1 (DNMT1) proteasomal degradation.
    Authors: Estève Et al.
    J Biol Chem
  10. Phosphorylation and degradation of tomosyn-2 de-represses insulin secretion.
    Authors: Bhatnagar Et al.
    J Biol Chem
  11. Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex.
    Authors: Liang Et al.
    Cell Cycle
  12. Mammalian target of rapamycin complex 2 modulates αβTCR processing and surface expression during thymocyte development.
    Authors: Chou Et al.
    J Immunol
  13. BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria.
    Authors: Choubey Et al.
  14. Arkadia regulates tumor metastasis by modulation of the TGF-β pathway.
    Authors: Briones-Orta Et al.
    PLoS One
  15. A critical role for protein degradation in the nucleus accumbens core in cocaine reward memory.
    Authors: Ren Et al.
  16. Proteasomal degradation of eukaryotic elongation factor-2 kinase (EF2K) is regulated by cAMP-PKA signaling and the SCFβTRCP ubiquitin E3 ligase.
    Authors: Wiseman Et al.
    J Biol Chem
  17. Drosophila SOCS36E negatively regulates JAK/STAT pathway signaling via two separable mechanisms.
    Authors: Stec Et al.
    Mol Biol Cell
  18. Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK.
    Authors: Shrestha and Draviam
    Curr Biol
  19. Human bocavirus VP2 upregulates IFN-β pathway by inhibiting ring finger protein 125-mediated ubiquitination of retinoic acid-inducible gene-I.
    Authors: Luo Et al.
    J Immunol
  20. Autophagy-mediated turnover of dynamin-related protein 1.
    Authors: Purnell and Fox
    BMC Neurosci
  21. Automated tracking of mitotic spindle pole positions shows that LGN is required for spindle rotation but not orientation maintenance.
    Authors: Corrigan Et al.
    Eur J Neurosci
  22. Spatiotemporal organization of Aurora-B by APC/CCdh1 after mitosis coordinates cell spreading through FHOD1.
    Authors: Floyd Et al.
    J Appl Physiol (1985)
  23. Molecular mechanism of mutant p53 stabilization: the role of HSP70 and MDM2.
    Authors: Wiech Et al.
    PLoS One
  24. Tgf-β induced Erk phosphorylation of smad linker region regulates smad signaling.
    Authors: Hough Et al.
    PLoS One
  25. Free fatty acid palmitate impairs the vitality and function of cultured human bladder smooth muscle cells.
    Authors: Oberbach Et al.
    Proc Natl Acad Sci U S A
  26. Reversible modification of adenomatous polyposis coli (APC) with K63-linked polyubiquitin regulates the assembly and activity of the β-catenin destruction complex.
    Authors: Tran and Polakis
    J Biol Chem
  27. Quantitative profiling of caspase-cleaved substrates reveals different drug-induced and cell-type patterns in apoptosis.
    Authors: Shimbo Et al.
    Stem Cells
  28. Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors.
    Authors: Bassett Et al.
    Sci Rep
  29. N-methyl-D-aspartate receptor- and metabotropic glutamate receptor-dependent long-term depression are differentially regulated by the ubiquitin-proteasome system.
    Authors: Citri Et al.
    Cancer Res
  30. Leptin enhances synthesis of proinflammatory mediators in human osteoarthritic cartilage--mediator role of NO in leptin-induced PGE2, IL-6, and IL-8 production.
    Authors: Vuolteenaho Et al.
    Mediators Inflamm
  31. The effect of denervation on protein synthesis and degradation in adult rat diaphragm muscle.
    Authors: Argadine Et al.
    J Exp Med
  32. Calcineurin-mediated dephosphorylation of c-Jun Ser-243 is required for c-Jun protein stability and cell transformation.
    Authors: Huang Et al.
  33. Effects of levo- and dextrosimendan on NF-kappaB-mediated transcription, iNOS expression and NO production in response to inflammatory stimuli.
    Authors: Sareila Et al.
    Br J Pharmacol
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