MHC Class I Antibody (F21-2) [FITC]

Novus Biologicals | Catalog # NBP1-28305

Novus Biologicals

Key Product Details

Species Reactivity

Avian, Chicken, Turkey

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # F21-2
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Product Specifications

Immunogen

A monomorphic epitope on avian MHC Class I molecules (Mr approx. 68 kDa)

Reactivity Notes

Chicken and Turkey

Specificity

Chicken/Turkey MHC Class I

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MHC Class I Antibody (F21-2) [FITC]

Flow Cytometry: MHC Class I Antibody (F21-2) [FITC] [NBP1-28305] - Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken MHC Class I-FITC.
Flow Cytometry: MHC Class I Antibody (F21-2) [FITC] [NBP1-28305] - 1 ug/10^6 Chicken peripheral blood mononuclear cells were double-stained with mouse anti-chicken MHC Class I-FITC and mouse anti-chicken IgM-PE. Small lymphocytes were then gated and analyzed on a flow cytometer

Applications for MHC Class I Antibody (F21-2) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MHC Class I

Like their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens) consist of a highly polymorphic alpha-chain (Mr approx. 44 kDa) non-covalently bound to the invariant beta2-microglobulin subunit (Mr approx. 12 kDa). MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (1-3)

Alternate Names

DKFZp686P19218, EA1.2, EA2.1, HLA class I histocompatibility antigen, alpha chain E, HLA class I histocompatibility antigen, E alpha chain, HLA-6.2, HLAE, lymphocyte antigen, major histocompatibility complex, class I, E, MHC, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, QA1

Gene Symbol

HLA-E

Additional MHC Class I Products

Product Documents for MHC Class I Antibody (F21-2) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MHC Class I Antibody (F21-2) [FITC]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for MHC Class I Antibody (F21-2) [FITC]

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  • Q: Has this product, NBP1-28305, been tested for cross reactivity with human MHC class I or any other human cell surface markers?

    A: NBP1-28305 is not tested for any cross-reactivity against human. For human MHC class I, I would recommend our product NB100-64212.

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