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If you are interested in more 3-D culture and organoid recipes from R&D Systems, check out our organoid resource and reagent guide.
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R&D Systems continues to support research studying adult epithelial stem cells including their culture, expansion, and differentiation in both 2-D and 3-D culture platforms. To further support your work in this field, we are proud to provide recipes for the culture and expansion of adult epithelial stem cells and differentiation recipes for the gastrointestinal tract and airway system based on current published literature.
Adult Epithelial Stem Cell Expansion Media
This recipe has been validated for use in the culture of adult stem cells from the gastrointestinal tract and airway systems.
| Cultrex® Basement Membrane Extracts and Extracellular Matrices |
| 3T3-J2 Mouse Irradiated Feeder Cells |
| 6-well tissue culture plates |
| Wang, X. et al. (2015) Nature 522:173. |
| Duleba, M. et al. (2018) Gastroenterology 10:1053. |
Differentiation Media for Adult Intestinal Stem Cells to Gastrointestinal Tissue in ALI Culture
This recipe can be used for the differentiation of adult intestinal stem cells into 3-D tissue using an air-liquid interface (ALI) culture system for all regions of the gastrointestinal tract.
| 3T3-J2 Mouse Irradiated Feeder Cells | |
| Cultrex® Basement Membrane Extracts and Extracellular Matrices | |
| Corning™ 3470 – 6.5mm Transwell® with 0.4 µm pore size polyester membrane inserts | |
| Recommended Markers | |
|---|---|
| Muc2 (at novusbio.com) | |
| Villin | |
| CDH17 | |
| SOX9 | |
| CHGA | |
| DEF6A (at novusbio.com) | |
| References: | |
|---|---|
| Wang, X. et al. (2015) Nature 522:173. | |
| Duleba, M. et al. (2018) Gastroenterology 10:1053. | |
Differentiation Media for Adult Epithelial Stem Cells to Airway Tissue in ALI Culture
This recipe can be used for the differentiation of adult stem cells from airway tissues into 3-D tissue using an air-liquid interface culture system for all regions of the airway system.
| Cultrex® Basement Membrane Extracts and Extracellular Matrices |
| 3T3-J2 Mouse Irradiated Feeder Cells |
| Corning™ 3470 – 6.5mm Transwell® with 0.4 µm pore size polyester membrane inserts |
| Kumar, P. et al. (2011) Cell 147:525. |
| Yamamoto, Y. et al. (2015) Nature Communications 7:10380. |