MimEX™ Tissue Model Systems is a next-generation 3-dimensional (3-D) cell culture platform for the generation of ex vivo epithelial-derived organ tissue.
This transformative technology harnesses the unique characteristics of adult ground-state epithelial stem cells to generate ex vivo organ tissue that recapitulates native cytoarchitecture and mimics physiological attributes of the desired organ. Consisting of optimized media and reagents, MimEX™ Tissue Model Systems provides an alternative to current 3-D cell culture modes, such as organoids, with the added benefit of better tissue access, control of tissue variability, and regional and disease tissue specificity.
Generate 3-D Epithelial Organ Tissue
MimEX™ Media and Reagents support the expansion and differentiation of adult stem cells from epithelial-derived organs. Learn more about what MimEX™ Tissue Model Systems has to offer by selecting a tissue below.
The gastrointestinal tract is currently the only organ system available in the MimEX™ System.
View MimEX™ GI Portfolio
MimEX™ Airway is under development. This system will enable the generation of 3-D tissue from specific subregions of the airway tract, including proximal and distal lung tissue.
The technology behind MimEX™ Tissue Model Systems applies to other epithelial-derived organs and tissue, including liver, skin, kidney, prostate, ovary, and pancreas.
Visit our webpage on Organoid and 3-D Cell Culture Research for more tools for 3-D cell culture.
Overview of the MimEX™ Technology
Tissue-specific Adult Stem Cells
The power of MimEX™ Tissue Models arises from the unique characteristics of adult ground-state stem cells, which retain the ability to grow into organ tissue ex vivo. Using MimEX™ Reagents, adult stem cells from healthy or diseased epithelial tissue can be isolated, expanded, and differentiated to generate a 3-D cell culture model for use in studying disease, drug screening*, toxicology testing, and basic developmental biology studies.
MimEX™ Expansion Media
Cryopreserved or freshly-isolated adult epithelial stem cells can be grown in MimEX™ Expansion Media without compromising their clonogenic potential or genetic stability. After expansion, transition the cells into an air-liquid interface to generate 3-D organ tissue.
MimEX™ Differentiation Media
Tissue-specific adult stem cells differentiate into 3-D tissue when grown in an air-liquid interface with MimEX™ Differentiation Media. This 3-D system mimics the native tissue cytoarchitecture, preserves cell-cell interactions, and provides access to the luminal surface of the tissue.
Benefits of MimEX™ Tissue Model Systems
In addition to generating tissue that mimics the cytoarchitecture and physiological attributes of native tissue, MimEX™ Tissue Systems addresses the limitations and challenges of current 3-D cell culture models, such as organoids and spheroids.
MimEX™ Media and Reagents generate 3-D tissue on a 2-D interface, creating an open-faced organoid, exposing the epithelial surface of the tissue. This provides better access to the functional surface of the 3-D tissue, compared to current organoid models.
Tissue generated using MimEX™ Media and Reagents arises from a monolayer of tissue-specific adult stem cells. This provides greater control over the size and shape of the 3-D tissue, which can be variable in organoid and 3-D cell culture models.
The power of MimEX™ Tissue Models is in the region-specific memory of adult ground-state stem cells. These cells, when isolated from an organ or subregion of an organ, can be expanded in culture and will differentiate back into functional region-specific tissue.
Preservation of Diseased Tissue
Adult stem cells, isolated from pathogenic tissue, differentiate back into the diseased tissue ex vivo. Because of this attribute, tissue generated with MimEX™ Reagents can be utilized for evaluating disease progression, biomarker discovery, drug screening, and toxicology testing.
Clonal Expansion and Gene Editing
MimEX™ Expansion Media enables the clonal propagation of tissue-specific adult stem cells. Individual cells can be isolated and robustly expanded in vitro, making MimEX™ Tissue accessible to gene editing techniques, such as CRISPR-Cas9.
MimEX™ Tissue Model Systems provides an accessible and sustainable ex vivo platform that is ideal for disease modeling, drug screening, toxicology testing, and basic developmental biology studies.
A Novel 3-D Cell Culture Platform for Epithelial Tissues
The technology behind MimEX™ Tissue Model Systems is applicable for the expansion and differentiation of adult stem cells from epithelial organs, such as the gastrointestinal tract, airway system, liver, kidney, and more. Learn more about the potential of MimEX™ Technology by downloading our free guide.
The gastrointestinal tract is currently the only organ system available in the MimEX™ System. In vitro 3-D models of gastric and intestinal tissue are being used to study Crohn’s disease, irritable bowel syndrome, the microbiome, gastrointestinal cancers and metastasis, as well as drug toxicity, absorption, and metabolism. Learn more about MimEX™ GI Tissue.
MimEX™ Tissue Model Systems is optimized for the isolation, expansion, and differentiation of adult stem cells from epithelial-derived tissues, including airway, liver, skin, kidney, prostate, ovary, and pancreas tissue. Fill out the form and download our free guide to learn more about the potential applications for the MimEX™ technology.
Fill out the form for a free download of the MimEX™ System Guide
* Portions of this product are covered by a license to intellectual property owned by, or licensed to, Multiclonal Therapeutics, Inc. The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right under these intellectual property rights to use this product for research purposes only. The purchase of this product does not include any right to use this product or components of this product for resale, for drug discovery intended for commercial gain, for the generation of cells or tissues intended for transplantation in human patients, or for any other commercial purposes, including without limitation, provision of services to a third party, generation of commercial databases, or as a diagnostic or therapeutic. Multiclonal Therapeutics, Inc., reserves all other rights under these rights. For information on purchasing a license to the intellectual property for uses other than in conjunction with this product or to use this product for purposes other than research, please contact Multiclonal Therapeutics, Inc. at firstname.lastname@example.org.
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