Mineralocorticoid R/NR3C2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-70640

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Primate

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Mineralocorticoid R/NR3C2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to NR3C2(nuclear receptor subfamily 3, group C, member 2) The peptide sequence was selected from the middle region of NR3C2. Peptide sequence RRKNCPACRLQKCLQAGMNLGARKSKKLGKLKGIHEEQPQQQQPPPPPPP. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 23592886).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Mineralocorticoid R/NR3C2 Antibody - BSA Free

Western Blot: Mineralocorticoid R/NR3C2 Antibody [NBP1-70640]

Western Blot: Mineralocorticoid R/NR3C2 Antibody [NBP1-70640]

Western Blot: Mineralocorticoid R/NR3C2 Antibody [NBP1-70640] - Antibody Titration: 1. 25 ug/ml Positive control: A172 cell lysate NR3C2 is supported by BioGPS gene expression data to be expressed in A172.
Immunohistochemistry-Paraffin: Mineralocorticoid R/NR3C2 Antibody [NBP1-70640]

Immunohistochemistry-Paraffin: Mineralocorticoid R/NR3C2 Antibody [NBP1-70640]

Immunohistochemistry-Paraffin: Mineralocorticoid R/NR3C2 Antibody [NBP1-70640] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Simple Western: Mineralocorticoid R/NR3C2 Antibody [NBP1-70640]

Simple Western: Mineralocorticoid R/NR3C2 Antibody [NBP1-70640]

Simple Western: Mineralocorticoid R/NR3C2 Antibody [NBP1-70640] - Simple Western lane view shows lysates of A172. A specific band was detected for Mineralocorticoid R/NR3C2 at approximately 107 kDa (as indicated) using 20 ug/mL of Mineralocorticoid R/NR3C2.

Applications for Mineralocorticoid R/NR3C2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml
Application Notes
See Simple Western Antibody Database for Simple Western validation

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mineralocorticoid R/NR3C2

NR3C2 is a receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. It binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.

Long Name

Mineralocorticoid Receptor

Alternate Names

MineralocorticoidR, MLR, NR3C2

Entrez Gene IDs

4306 (Human)

Gene Symbol

NR3C2

UniProt

P08235 (Human)

Additional Mineralocorticoid R/NR3C2 Products

Product Documents for Mineralocorticoid R/NR3C2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mineralocorticoid R/NR3C2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Mineralocorticoid R/NR3C2 Antibody - BSA Free

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Protocols

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FAQs

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