Mitochondrial ribonuclease P protein 3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12679

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Mitochondrial ribonuclease P protein 3 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human Mitochondrial ribonuclease P protein 3 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for Mitochondrial ribonuclease P protein 3 Antibody - BSA Free

Western Blot: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679]

Western Blot: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679]

Western Blot: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679] - Lane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:67 kDaObserved band size:67 kDa
Immunoprecipitation: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679]

Immunoprecipitation: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679]

Immunoprecipitation: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679] - Lane A:0.5 mg 293 Whole Cell Lysate4 uL anti-MRPP3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MRPP3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 67 kDaObserved band size :67 kDa
Mitochondrial ribonuclease P protein 3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679] -

Immunocytochemistry/Immunofluorescence: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679] - Staining of MRPP3 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12679 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for Mitochondrial ribonuclease P protein 3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

5-10uL/mg of uLysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mitochondrial ribonuclease P protein 3

Mitochondrial ribonuclease P protein 3 functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends

Alternate Names

KIAA0391, mitochondrial ribonuclease P protein 3, MRPP3Mitochondrial RNase P protein 3

Gene Symbol

PRORP

Additional Mitochondrial ribonuclease P protein 3 Products

Product Documents for Mitochondrial ribonuclease P protein 3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mitochondrial ribonuclease P protein 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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