MitoMark Red I New

Biological Activity

MitoMark Red I-labeled HeLa cells

Application of MitoMark Red I: MitoMark Red I was diluted in HBSS and applied to live HeLa cells at a concentration of 200 nM. HeLa cells were incubated for 30 minutes at 37�C. 10% formalin was added to the HBSS and MitoMark solution at a ratio of 1:1 for 20 minutes at room temperature. After fixation, the cells were permeabilized using antibody diluent (1X PBS, 0.3% Triton X-100, 1% NDS, 1% BSA, and 0.01% NaN3) for 30 minutes at room temperature. Lastly, mounting media with DAPI (Fluoroshield� with DAPI from Sigma) was applied to the cells. Images represent a bright-field DIC to see cell boundaries combined with fluorescence.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Analysis of mitochondrial morphology and function with novel fixable fluorescent stains.
   Poot et al.
   J.Histochem.Cytochem., 1996;44:1363
2. MitoTracker labeling in primary neuronal and astrocytic cultures: influence of mitochondrial membrane potential and oxidants.
   Buckman et al.
   J.Neuroscience Methods., 2001;104:165

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