MLH1 Antibody (S02-2E8)

Name: MLH1 Antibody (S02-2E8)
Brand: Novus Biologicals
SKU: NBP3-19527
Price: 559 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-19527

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-2E8 expressed in HEK293

View all MLH-1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human MLH1 (Uniprot # P40692)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MLH1 Antibody (S02-2E8)

Western Blot: MLH1 Antibody (S02-2E8) [NBP3-19527]

Western Blot: MLH1 Antibody (S02-2E8) [NBP3-19527] - Western blot detection of MLH1 in K562, C6, 3T3, Hela cell lysates using NBP3-19527 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.

Immunocytochemistry/Immunofluorescence: MLH1 Antibody (S02-2E8) [NBP3-19527] -

Immunocytochemistry/Immunofluorescence: MLH1 Antibody (S02-2E8) [NBP3-19527] - Immunofluorescence of MLH1 in Hela cells using NBP3-19527 at dilution 1/200, and DAPI (blue)

Applications for MLH1 Antibody (S02-2E8)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Western Blot

1/2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MLH-1

MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPCC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

Long Name

mutL Homolog 1

Alternate Names

MLH1

Gene Symbol

MLH1

Additional MLH-1 Products

Product Documents for MLH1 Antibody (S02-2E8)

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Certificate of Analysis

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Product Specific Notices for MLH1 Antibody (S02-2E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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