Mouse CCL20/MIP-3 alpha APC-conjugated Antibody

Name: Mouse CCL20/MIP-3 alpha APC-conjugated Antibody
Brand: R&D Systems
SKU: IC760A

Catalog #: IC760A Datasheet / COA / SDS

Discontinued Product

IC760A has been discontinued.

View all CCL20/MIP-3 alpha products.

Detection of CCL20/MIP‑3 alpha in Mouse Splenocytes by Flow Cytometry.

1 Image

All CCL20/MIP-3 alpha Antibodies

Product Details

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL20/MIP-3 alpha APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL20/MIP‑3 alpha in direct ELISAs and Western blots. In direct ELISAs, 12% cross-reactivity with recombinant rat (rr) CCL20, 6% cross-reactivity with recombinant mouse (rm) CCL12 and recombinant human (rh) CCL25, and 3% cross‑reactivity with with rmCCL9/10/MIP-1 gamma is observed. No cross-reactivity with recombinant cotton rat CCL3, 4, 5, rhCCL1, 2, 3, 4, 5, 7, 8, 11,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, 27, 28, rmCCL1, 2, 3, 4, 5, 6, 7, 8, 11, 17, 19, 21, 22, 24, 25, 27, or 28 is observed.

Source

Monoclonal Rat IgG₁ Clone # 114906

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CCL20/MIP-3 alpha
Ala28-Met97
Accession # O89093

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of CCL20/MIP-3a antibody in Mouse Splenocytes antibody by Flow Cytometry.

View Larger

Detection of CCL20/MIP‑3 alpha in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) untreated or (B) treated with LPS were stained with Rat Anti-Mouse CCL20/MIP-3a APC-conjugated Monoclonal Antibody (Catalog # IC760A). Quadrant markers were set based on control antibody staining (Catalog # IC005A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCL20/MIP-3 alpha

CCL20, also known as MIP-3 alpha, LARC, and Exodus, is a beta chemokine. Mouse CCL20 cDNA encodes a 97 amino acid precursor protein with a 27 aa putative signal peptide that is predicted to be cleaved to form the 70 aa mature secreted protein. CCL20 is distantly related to other beta chemokines (20-28% aa sequence identity). Mouse CCL20 shares approximately 71 and 63% amino acid sequence homology with rat and human CCL20, respectively. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of CCL20 is strongly upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL-10. CCL20 has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. CCL20 is a functional ligand for CCR6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34⁺ cord blood precursors.

References

Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
Hromas, R. et al. (1997) Blood 89:3315.
Greaves, D. R. et al. (1997) J. Exp. Med. 186: 857.
Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.

Entrez Gene IDs

6364 (Human); 20297 (Mouse); 29538 (Rat)

Alternate Names

beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38