Mouse CD300f/LMIR3 Antibody

Detection of Monkey CD300f/LMIR3 by Immunocytochemistry/Immunofluorescence CLM-1 receptor expression levels are modified during microglial activation by TLRs agonists.A) Microglia was treated with LPS (100 ng/mL) at different time points (left) and with TLRs agonists LPS (TLR4 agonist, 100 ng/mL), PGN (TLR2/6 heterodimeragonist, 1 μg/mL) and poly I:C (TLR3 agonist, 10 μg/mL) for 24 hours (right). CLM-1 mRNA levels were quantified by QT-PCR. B) Surface expression of CLM-1 in microglial cells was monitored by flow cytometry 24 and 48 hours after treatment with LPS (100 ng/mL) or PGN (1μg/mL). Isotypic antibody (grey histograms), anti-CLM1 (white histograms). C) Immunostaining with an antibody for CLM-1 of microglia 24 hours after treatment with LPS (100 ng/mL) or PGN (1 μg/mL). Bar, 20 μM. Data are presented as mean ± SEM of 3 independent experiments. Statistically significant differences between treatments were determined by a one-way Anova followed by Newman Keules post-test. *P < 0.05 compared to IgG. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25927603), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Monkey CD300f/LMIR3 by Immunocytochemistry/Immunofluorescence CLM-1 mRNA and protein levels in microglial cells under basal conditions.A) QT-PCR analysis of CLM-1 transcript was performed on primary microglia total RNA. RAW264.7 and NIH3T3 cell lines were used as positive and negative controls respectively. CLM-1 transcript was normalized with 18S RNA levels. B) Surface expression of CLM-1 on primary microglia was monitored by flow cytometry using anti-CLM-1 mAb (white histogram) and an isotypic mAb as a negative control (gray histrogram). C) Immunohistochemistry on microglial primary cultures was performed to analyze the expression of CLM-1 in basal conditions, using an anti-CLM-1 monoclonal antibody. Nuclei were stained with DAPI. No staining was observed in the absence of primary antibody. Scale bar, 20 μM. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25927603), licensed under a CC-BY license. Not internally tested by R&D Systems.