Mouse CD300f/LMIR3 Antibody

R&D Systems | Catalog # MAB27881

R&D Systems
Discontinued Product
MAB27881 has been discontinued. View all CD300f/LMIR3 products.

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 305835
View all CD300f/LMIR3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD300f/LMIR3
Cys16-Gly188
Accession # Q6SJQ7

Specificity

Detects mouse CD300f/LMIR3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) LMIR4, rmCD300b/LMIR5, recombinant human (rh) CD300a/LMIR1, rhCD300c/LMIR2, rhCD300f/LMIR3, or rhCD300e/LMIR6 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse CD300f/LMIR3 Antibody

Detection of Monkey CD300f/LMIR3 by Immunocytochemistry/Immunofluorescence

Detection of Monkey CD300f/LMIR3 by Immunocytochemistry/Immunofluorescence

CLM-1 receptor expression levels are modified during microglial activation by TLRs agonists.A) Microglia was treated with LPS (100 ng/mL) at different time points (left) and with TLRs agonists LPS (TLR4 agonist, 100 ng/mL), PGN (TLR2/6 heterodimeragonist, 1 μg/mL) and poly I:C (TLR3 agonist, 10 μg/mL) for 24 hours (right). CLM-1 mRNA levels were quantified by QT-PCR. B) Surface expression of CLM-1 in microglial cells was monitored by flow cytometry 24 and 48 hours after treatment with LPS (100 ng/mL) or PGN (1μg/mL). Isotypic antibody (grey histograms), anti-CLM1 (white histograms). C) Immunostaining with an antibody for CLM-1 of microglia 24 hours after treatment with LPS (100 ng/mL) or PGN (1 μg/mL). Bar, 20 μM. Data are presented as mean ± SEM of 3 independent experiments. Statistically significant differences between treatments were determined by a one-way Anova followed by Newman Keules post-test. *P < 0.05 compared to IgG. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25927603), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Monkey CD300f/LMIR3 by Immunocytochemistry/Immunofluorescence

Detection of Monkey CD300f/LMIR3 by Immunocytochemistry/Immunofluorescence

CLM-1 mRNA and protein levels in microglial cells under basal conditions.A) QT-PCR analysis of CLM-1 transcript was performed on primary microglia total RNA. RAW264.7 and NIH3T3 cell lines were used as positive and negative controls respectively. CLM-1 transcript was normalized with 18S RNA levels. B) Surface expression of CLM-1 on primary microglia was monitored by flow cytometry using anti-CLM-1 mAb (white histogram) and an isotypic mAb as a negative control (gray histrogram). C) Immunohistochemistry on microglial primary cultures was performed to analyze the expression of CLM-1 in basal conditions, using an anti-CLM-1 monoclonal antibody. Nuclei were stained with DAPI. No staining was observed in the absence of primary antibody. Scale bar, 20 μM. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25927603), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CD300f/LMIR3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse CD300f/LMIR3

Reviewed Applications

Read 1 review rated 4 using MAB27881 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD300f/LMIR3

Mouse LMIR3, also known as CLM-1, IgSF13, IREM-1 and CMRF35-like molecule 1, and designated CD300f, is a 55 kDa type I transmembrane (TM) glycoprotein. It is synthesized as a 283 amino acid (aa) precursor that contains a putative 19 aa signal sequence, a 175 aa extracellular region that contains one Ig-like domain, a 17 aa TM segment and a 72 aa cytoplasmic domain that contains an ITIM sequence. There are multiple alleles for this gene that vary considerably in size. Mouse LMIR3 is expressed in monocytic cell lines. Mature mouse LMIR3 extracellular region shares 43% and 72% aa identity with human and rat LMIR3 extracellular region, respectively.

Long Name

Leukocyte Mono Ig-like Receptor 3

Alternate Names

CD300f, CD300LF, CLIM1, CLM1, DIgR2, IGSF13, IREM-1, LMIR3, NKIR, Pigr3

Entrez Gene IDs

146722 (Human); 246746 (Mouse)

Gene Symbol

CD300LF

UniProt

Q6SJQ7

Additional CD300f/LMIR3 Products

Product Documents for Mouse CD300f/LMIR3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD300f/LMIR3 Antibody

For research use only

Citations for Mouse CD300f/LMIR3 Antibody

Customer Reviews for Mouse CD300f/LMIR3 Antibody (1)

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  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 21951326
    Species: Rat
    Verified Customer | Posted 02/19/2015

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