Mouse CD300f/LMIR3 APC-conjugated Antibody

FAB27881A has been discontinued. View all CD300f/LMIR3 products.
    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD300f/LMIR3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) LMIR4, rmCD300b/LMIR5, recombinant human (rh) CD300a/LMIR1, rhCD300c/LMIR2, rhCD300f/LMIR3, or rhCD300e/LMIR6 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 305835
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD300f/LMIR3
    Cys16-Gly188
    Accession # Q6SJQ7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD300f/LMIR3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CD300f/LMIR3 APC‑conjugated Monoclonal Antibody (Catalog # FAB27881A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD300f/LMIR3
Mouse LMIR3, also known as CLM-1, IgSF13, IREM-1 and CMRF35-like molecule 1, and designated CD300f, is a 55 kDa type I transmembrane (TM) glycoprotein. It is synthesized as a 283 amino acid (aa) precursor that contains a putative 19 aa signal sequence, a 175 aa extracellular region that contains one Ig-like domain, a 17 aa TM segment and a 72 aa cytoplasmic domain that contains an ITIM sequence. There are multiple alleles for this gene that vary considerably in size. Mouse LMIR3 is expressed in monocytic cell lines. Mature mouse LMIR3 extracellular region shares 43% and 72% aa identity with human and rat LMIR3 extracellular region, respectively.
  • Long Name:
    Leukocyte Mono Ig-like Receptor 3
  • Entrez Gene IDs:
    146722 (Human); 246746 (Mouse)
  • Alternate Names:
    CMRF35-like molecule 1; CD300 molecule-like family member f; CD300f; CD300LF; CLIM1; CLM1; CLM-1; DIgR2; IGSF13; IREM1; IREM-1; LMIR3; NKIR; Pigr3
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