Home / CD300f/LMIR3 / Mouse CD300f/LMIR3 APC-conjugated Antibody

Mouse CD300f/LMIR3 APC-conjugated Antibody

Catalog #: FAB27881A Datasheet / COA / SDS

Discontinued Product

FAB27881A has been discontinued.
View all CD300f/LMIR3 products.
Detection of CD300f/LMIR3 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
1 Image
Product Details
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD300f/LMIR3 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD300f/LMIR3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) LMIR4, rmCD300b/LMIR5, recombinant human (rh) CD300a/LMIR1, rhCD300c/LMIR2, rhCD300f/LMIR3, or rhCD300e/LMIR6 is observed.
Source
Monoclonal Rat IgG1 Clone # 305835
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD300f/LMIR3
Cys16-Gly188
Accession # Q6SJQ7
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD300f/LMIR3 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CD300f/LMIR3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CD300f/LMIR3 APC-conjugated Monoclonal Antibody (Catalog # FAB27881A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD300f/LMIR3

Mouse LMIR3, also known as CLM-1, IgSF13, IREM-1 and CMRF35-like molecule 1, and designated CD300f, is a 55 kDa type I transmembrane (TM) glycoprotein. It is synthesized as a 283 amino acid (aa) precursor that contains a putative 19 aa signal sequence, a 175 aa extracellular region that contains one Ig-like domain, a 17 aa TM segment and a 72 aa cytoplasmic domain that contains an ITIM sequence. There are multiple alleles for this gene that vary considerably in size. Mouse LMIR3 is expressed in monocytic cell lines. Mature mouse LMIR3 extracellular region shares 43% and 72% aa identity with human and rat LMIR3 extracellular region, respectively.

Long Name
Leukocyte Mono Ig-like Receptor 3
Entrez Gene IDs
146722 (Human); 246746 (Mouse)
Alternate Names
CMRF35-like molecule 1; CD300 molecule-like family member f; CD300f; CD300LF; CLIM1; CLM1; CLM-1; DIgR2; IGSF13; IREM1; IREM-1; LMIR3; NKIR; Pigr3

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse CD300f/LMIR3 APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse CD300f/LMIR3 APC-conjugated Antibody and earn rewards!

Have you used Mouse CD300f/LMIR3 APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review