Mouse CD300f/LMIR3 APC‑conjugated Antibody

R&D Systems | Catalog # FAB27881A

R&D Systems
Discontinued Product
FAB27881A has been discontinued. View all CD300f/LMIR3 products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 305835
View all CD300f/LMIR3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD300f/LMIR3
Cys16-Gly188
Accession # Q6SJQ7

Specificity

Detects mouse CD300f/LMIR3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) LMIR4, rmCD300b/LMIR5, recombinant human (rh) CD300a/LMIR1, rhCD300c/LMIR2, rhCD300f/LMIR3, or rhCD300e/LMIR6 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse CD300f/LMIR3 APC‑conjugated Antibody

Detection of CD300f/LMIR3 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of CD300f/LMIR3 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CD300f/LMIR3 APC-conjugated Monoclonal Antibody (Catalog # FAB27881A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD300f/LMIR3 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD300f/LMIR3

Mouse LMIR3, also known as CLM-1, IgSF13, IREM-1 and CMRF35-like molecule 1, and designated CD300f, is a 55 kDa type I transmembrane (TM) glycoprotein. It is synthesized as a 283 amino acid (aa) precursor that contains a putative 19 aa signal sequence, a 175 aa extracellular region that contains one Ig-like domain, a 17 aa TM segment and a 72 aa cytoplasmic domain that contains an ITIM sequence. There are multiple alleles for this gene that vary considerably in size. Mouse LMIR3 is expressed in monocytic cell lines. Mature mouse LMIR3 extracellular region shares 43% and 72% aa identity with human and rat LMIR3 extracellular region, respectively.

Long Name

Leukocyte Mono Ig-like Receptor 3

Alternate Names

CD300f, CD300LF, CLIM1, CLM1, DIgR2, IGSF13, IREM-1, LMIR3, NKIR, Pigr3

Entrez Gene IDs

146722 (Human); 246746 (Mouse)

Gene Symbol

CD300LF

UniProt

Q6SJQ7

Additional CD300f/LMIR3 Products

Product Documents for Mouse CD300f/LMIR3 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD300f/LMIR3 APC‑conjugated Antibody

For research use only

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