Mouse CLEC9a APC‑conjugated Antibody

R&D Systems | Catalog # FAB67761A

R&D Systems
Discontinued Product
FAB67761A has been discontinued. View all CLEC9a products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 7H11
View all CLEC9a Primary Antibodies »

Product Specifications

Immunogen

RBL‑2H3 rat basophilic leukemia cell line expressing mouse CLEC9a fused to an HA epitope
Accession # Q8BRU4

Specificity

Detects mouse CLEC9a in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse CLEC9a APC‑conjugated Antibody

Detection of CLEC9a antibody in Mouse Splenoctyes antibody by Flow Cytometry.

Detection of CLEC9a in Mouse Splenoctyes by Flow Cytometry.

CD4-CD8+mouse splenocytes were stained with Hamster Anti-Mouse CD11c PE-conjugated Monoclonal Antibody (Catalog # FAB69501P) and either (A) Rat Anti-Mouse CLEC9a APC-conjugated Monoclonal Antibody (Catalog # FAB67761A) or (B) Rat IgG1Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CLEC9a APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: CD4- CD8+ mouse splenocytes

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CLEC9a

CLEC9a (C-type Lectin domain family 9 member A), also known as DNGR-1, is a 44-48 kDa type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as either a disulfide-linked homodimer or monomer. Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes. In mouse, the human BDCA-3+ cDC are analogous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In addition, mouse CLEC9a is also expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I molecules and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk, indicating that this is an activating receptor. Alternative splicing of mouse CLEC9a generates isoforms with deletions in the transmembrane segment, stalk region, or CTLD. Within aa 57-264 of the ECD, mouse CLEC9a shares 57% and 80% aa sequence identity with human and rat CLEC9a, respectively.

Long Name

C-type Lectin-like Receptor 9

Alternate Names

CD370, DNGR1

Entrez Gene IDs

283420 (Human); 232414 (Mouse)

Gene Symbol

CLEC9A

UniProt

Q8BRU4 (Mouse)

Additional CLEC9a Products

Product Documents for Mouse CLEC9a APC‑conjugated Antibody

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Product Specific Notices for Mouse CLEC9a APC‑conjugated Antibody

For research use only

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail