Detection of CLEC9a in Mouse Splenoctyes by Flow Cytometry. CD4- CD8+ mouse splenocytes were stained with Hamster Anti-Mouse CD11c PE‑conjugated Monoclonal Antibody (Catalog # FAB69501P) and either (A) Rat Anti-Mouse CLEC9a APC‑conjugated Monoclonal Antibody (Catalog # FAB67761A) or (B) Rat IgG1 Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CLEC9a (C-type Lectin domain family 9 member A), also known as DNGR-1, is a 44-48 kDa type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as either a disulfide-linked homodimer or monomer. Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes. In mouse, the human BDCA-3+ cDC are analogous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In addition, mouse CLEC9a is also expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I molecules and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk, indicating that this is an activating receptor. Alternative splicing of mouse CLEC9a generates isoforms with deletions in the transmembrane segment, stalk region, or CTLD. Within aa 57-264 of the ECD, mouse CLEC9a shares 57% and 80% aa sequence identity with human and rat CLEC9a, respectively.
C-type Lectin-like Receptor 9
Entrez Gene IDs:
283420 (Human); 232414 (Mouse)
CD370; CLEC9a; C-type lectin domain family 9 member A; C-type lectin domain family 9, member A; DNGR1; HEEE9341; UNQ9341
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