Detection of CXCR7/RDC‑1 in D3 Mouse Cell Line by Flow Cytometry.
D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CXCR7/RDC‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB7167A, filled histogram) or isotype control antibody rat Igm isotype control. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CXCR7 (CXC chemokine receptor 7; also GPRN1, RDC-1 and chemokine orphan receptor 1) is a 60 kDa member of the G-protein coupled receptor 1 family. It is expressed on multiple cell types, including neurons, T cells, NK cells, neutrophils, B cells plus angiogenic endothelial cells. CXCR7 forms both homodimers and heterodimers with CXCR4. It selectively binds I-TAC and SDF-1, and appears to involve beta -arrestin2 during signaling. Notably, a CXCR7:CXCR4 heterodimer shows increased responsiveness to SDF-1, and I-TAC may actually block some SDF-1-mediated migration activity. Mouse CXCR7 is a 7-transmembrane glycoprotein that is 362 amino acids (aa) in length. It contains a 47 aa N-terminal extracellular region plus a 43 aa C-terminal cytoplasmic domain. Over the collective extracellular domains (aa 1-47, 103-118, 184-213 and 274-296), mouse CXCR7 shares 97% and 91% aa identity with rat and human CXCR7, respectively.
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