|Detection of Dtk in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line untreated (light orange filled histogram) or treated with Retinoic Acid for 48 hours (dark orange filled histogram) was stained with Rat Anti-Mouse Dtk PE‑conjugated Monoclonal Antibody (Catalog # FAB759P) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.|
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.
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