Mouse F4/80 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5580P-100UG
Mouse F4/80 PE-conjugated Antibody in Flow Cytometry
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Product Details
Citations (3)
FAQs
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Mouse F4/80 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse F4/80/EMR1.
Source
Monoclonal Rat IgG2A Clone # 521204
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. View Larger

Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5580P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: F4/80

F4/80, also known as EMR1 in humans, is a 160 kDa glycosylated protein that belongs to the EGF-TM7 family of adhesion molecules. It is expressed on splenic red pulp and bone marrow, macrophages, microglia, Langerhans cells, monocytes, eosinophils, and CD116+ dendritic cells in mouse, but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance, being necessary for the development of CD8+ T regs. It also serves as a marker for IL-1 beta + proinflammatory macrophages.

Entrez Gene IDs
13733 (Mouse); 316137 (Rat)
Alternate Names
ADGRE1; Adhesion G protein-coupled receptor E1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; Mouse EMR1; Rat EMR1; TM7LN3

Product Datasheets

Citations for Mouse F4/80 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Major vault protein suppresses obesity and atherosclerosis through inhibiting IKK-NF-?B signaling mediated inflammation
    Authors: J Ben, B Jiang, D Wang, Q Liu, Y Zhang, Y Qi, X Tong, L Chen, X Liu, Y Zhang, X Zhu, X Li, H Zhang, H Bai, Q Yang, J Ma, EAC Wiemer, Y Xu, Q Chen
    Nat Commun, 2019;10(1):1801.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. IL-12 promotes myeloid-derived suppressor cell recruitment and bacterial persistence during Staphylococcus aureus orthopedic implant infection.
    Authors: Heim C, Vidlak D, Scherr T, Hartman C, Garvin K, Kielian T
    J Immunol, 2015;194(8):3861-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Galectin-3 negatively regulates the frequency and function of CD4(+) CD25(+) Foxp3(+) regulatory T cells and influences the course of Leishmania major infection.
    Authors: Fermino M, Dias F, Lopes C, Souza M, Cruz A, Liu F, Chammas R, Roque-Barreira M, Rabinovich G, Bernardes E
    Eur J Immunol, 2013;43(7):1806-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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