Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 521204
View all F4/80 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549

Specificity

Detects mouse F4/80/EMR1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse F4/80 Antibody

Detection of F4/80/EMR1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 Monoclonal Antibody (Catalog # MAB5580, filled histogram) or isotype control antibody (MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

F4/80 in RAW 264.7 mouse monocyte/macrophage cell line.

F4/80 was detected in unfixed live cells fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB5580) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Applications for Mouse F4/80 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed and unfixed live RAW 264.7 mouse monocyte/macrophage cell line

Reviewed Applications

Read 2 reviews rated 3 using MAB5580 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: F4/80

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Alternate Names

DD7A5-7, Gpf480, Ly71

Entrez Gene IDs

13733 (Mouse); 316137 (Rat)

Gene Symbol

ADGRE1

UniProt

Q61549

Additional F4/80 Products

Product Documents for Mouse F4/80 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse F4/80 Antibody

For research use only

Citations for Mouse F4/80 Antibody

Customer Reviews for Mouse F4/80 Antibody (2)

3 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
50%

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Customer Images

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  • Mouse F4/80 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Liver
    Species: Mouse
    Verified Customer | Posted 06/05/2018
    Mouse F4/80 Antibody MAB5580
  • Mouse F4/80 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colon tissue
    Species: Mouse
    Verified Customer | Posted 12/01/2017
    We tried 4 difference sources and none of them, including this one, works. Our experience is that no F4/80 antibody works for FFPE samples. Although it is not uncommon to see published positive staining, those data using FFPE samples cannot be trusted.
    Bio-Techne Response
    Technical service is following up

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Protocols

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FAQs for Mouse F4/80 Antibody

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  • Q: Does this F4/80/EMR1 antibody come in lyophilized form?

    A: Yes, we carry this F4/80 antibody in lyophilized form.

  • Q: What is the immunogen sequence of this F4/80/EMR1 antibodies antibody?

    A: A sequence corresponding to Gln28 - Gly931 of mouse F4/80/EMR1.

  • Q: Does this F4/80/EMR1 antibody come in lyophilized form?

    A: Yes, we carry this F4/80 antibody in lyophilized form.

  • Q: What is the immunogen sequence of this F4/80/EMR1 antibodies antibody?

    A: A sequence corresponding to Gln28 - Gly931 of mouse F4/80/EMR1.

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